LOCUS       sequence-163428-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  receives miRNA target sequences for testing using the dual 
            luciferase assay system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Moyle RL, Carvalhais LC, Pretorius LS, Nowak E, Subramaniam G, 
            Dalton-Morgan J, Schenk PM
  TITLE     An Optimized Transient Dual Luciferase Assay for Quantifying 
            MicroRNA Directed Repression of Targeted Sequences.
  JOURNAL   Front Plant Sci. 2017 Sep 20;8:1631. doi: 10.3389/fpls.2017.01631. 
            eCollection 2017.
  PUBMED    28979287
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-09-20"; volume: "8:1631. doi"; pages: " 
            10.3389/fpls.2017.01631. eCollection 2017"
COMMENT     Sequence Label: pGrDL_SP
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..110
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(183..527)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(219..239)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(601..620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(602..620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(630..647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(630..646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(639..661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atttcgaagt actcagcgta agtgatgtcc acctcgatat gtgcatctgt aaaagcaatt
       61 gttccaggaa ccagggcgta tctcttcata gccttatgca gttgctctcc agcggttcca
      121 tcttccagcg gatagaatgg cgccgggcct ttctttatgt ttttggcgtc ttccatctcg
      181 aggtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
      241 gggattgtgc gtcatccctt acgtcagtgg agatgtcaca tcaatccact tgctttgaag
      301 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
      361 gaccactgtc ggcagaggca tcttgaatga tagcctttcc tttatcgcaa tgatggcatt
      421 tgtaggagcc accttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat
      481 ggaatccgag gaggtttccc gaaattaccc tttgttgaaa agtctcaata gccctttggt
      541 cttctgagac tgtatctttg acatttttgg agtagggggg tgggcccggt acccaattcg
      601 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
      661 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      721 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      781 gaatggaaat tgtaagcgtt aatgttatcg atcatgagcg gagaattaag ggagtcacgt
      841 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca
      901 acgttgaagg agccactgag ccgcgggttt ctggagttta a
//
