LOCUS       sequence-163432-       10705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRICK co-localization control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Voigt F, Eglinger J, Chao JA
  TITLE     Detection of the First Round of Translation: The TRICK Assay.
  JOURNAL   Methods Mol Biol. 2018;1649:373-384. doi: 
            10.1007/978-1-4939-7213-5_25.
  PUBMED    29130211
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1649:373-384. doi"; pages: " 
            10.1007/978-1-4939-7213-5_25"
COMMENT     Sequence Label: pSF4 TET CMV intron renilla 24xPP7 24xMS2
FEATURES             Location/Qualifiers
     source          1..10705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2263..2285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2385..2404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3716..3763)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3809..3827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3845..3863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3881..3899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3917..3935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3953..3971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3989..4007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4025..4043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4088..4112
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4189..4321
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4304..4323
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4366..4384
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4388..4397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4394..5329
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8498..8632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8576..8595
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8740..8773)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8826..8842)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(8858..8880)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9291..9648
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             9711..10304
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtcatcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaattcc
     2641 aagctaagtg aaagactctg tctcaaaaac caacctgaca cctgagccgg agcgttggct
     2701 ctcactacca tagaatattt tttcacaaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca cacagtcata cacatagaca cagtcacaca cacacacatg cacacacaca
     2821 cacacacact cacacacaca catacataca cacattcaca cacacccata cacacatttt
     2881 cacagtcaca cacacaaaca caatcacaca cacacacatc aatcaatcaa tcaatcaata
     2941 taccaaaaat gatagctcat acctgtaatc ttagatgtgg gaaggctaaa acagcaggct
     3001 acccatgact tcaaggtcac cctgggttac agagtagcta atagtctagc ttgaactata
     3061 gtgtaggatc tttaaaaaaa aattaaccaa ccaaccaaca aacaaacaaa caaacaacaa
     3121 caacaacaaa tggaagacag aggacaaaaa aaaataaaag aaaaagtaga aagccaatgg
     3181 agatactatc ttaaaattag aaatatttat gacacattct gaatactcca tgtttatttc
     3241 aaggaagcca atgtaacttt aagtactatg gccatttaat gtaaatactt aagaaaaaaa
     3301 accaaattaa ttttgataca tgctgcatgt gtgaaagacc cccgctgacg ggtagtcaat
     3361 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     3421 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     3481 tgaggggttg tgggctcgag ctcggggagc agaagcgcgc gaacagaagt cgagctagct
     3541 tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct
     3601 tttattttat ctatggctcg tactctatag gcttcagctg gtgatattgt tgagtcaaaa
     3661 ctagagcctg gaccactgat atcctgtctt taacaaattg gactaatccg gatctgaagt
     3721 tcctatactt tctagagaat aggaacttcg gaataggaac ttcgcggcag atcttttgcg
     3781 gccgctttgg gccggccgaa ttctttactc cctatcagtg atagagaacg tatgaagagt
     3841 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
     3901 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
     3961 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
     4021 ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata
     4081 aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt
     4141 cttataccaa ctttccgtac cacttcctac cctcgtaaaa ccggtcaggt aagtatcaag
     4201 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
     4261 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
     4321 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     4381 taggctagcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
     4441 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
     4501 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc
     4561 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
     4621 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
     4681 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
     4741 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa
     4801 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg
     4861 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct
     4921 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc
     4981 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac
     5041 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat
     5101 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga
     5161 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac
     5221 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg
     5281 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagtaag tcgacagatc
     5341 ctacctgcaa agcaccagag gatatggcct cggtgcctca gcatcttcag atgtgctgtc
     5401 actgtccaat cttcaagcca gcagagcata tgggctcgct ggctccactg tcattacagt
     5461 ggacctgatc ttctccaatc aaaggagcag aggatatggc ctcgctccct ccgactacat
     5521 caggctggaa gtgacatcca tcattcatcc cagcagagca tatgggctcg ctgggaccac
     5581 agcatgcctc gaagtgctga gagactatca tcaaaccgac agaggatatg gcctcgtcgg
     5641 ctcctactcc ctcctggtgg aatacttctc cactcttcaa cccagcagag catatgggct
     5701 cgctgggtcc atcagagata caagatctct cgacagatcc tacctgcaaa gcaccagagg
     5761 atatggcctc ggtgcctcag catcttcaga tgtgctgtca ctgtccaatc ttcaagccag
     5821 cagagcatat gggctcgctg gctccactgt cattacagtg gacctgatct tctccaatca
     5881 aaggagcaga ggatatggcc tcgctccctc cgactacatc aggctggaag tgacatccat
     5941 cattcatccc agcagagcat atgggctcgc tgggaccaca gcatgcctcg aagtgctgag
     6001 agactatcat caaaccgaca gaggatatgg cctcgtcggc tcctactccc tcctggtgga
     6061 atacttctcc actcttcaac ccagcagagc atatgggctc gctgggtcca tcagagatac
     6121 aagatctctc gacagatcct acctgcaaag caccagagga tatggcctcg gtgcctcagc
     6181 atcttcagat gtgctgtcac tgtccaatct tcaagccagc agagcatatg ggctcgctgg
     6241 ctccactgtc attacagtgg acctgatctt ctccaatcaa aggagcagag gatatggcct
     6301 cgctccctcc gactacatca ggctggaagt gacatccatc attcatccca gcagagcata
     6361 tgggctcgct gggaccacag catgcctcga agtgctgaga gactatcatc aaaccgacag
     6421 aggatatggc ctcgtcggct cctactccct cctggtggaa tacttctcca ctcttcaacc
     6481 cagcagagca tatgggctcg ctgggtccat cagagataca agatctctcg acagatccta
     6541 cctgcaaagc accagaggat atggcctcgg tgcctcagca tcttcagatg tgctgtcact
     6601 gtccaatctt caagccagca gagcatatgg gctcgctggc tccactgtca ttacagtgga
     6661 cctgatcttc tccaatcaaa ggagcagagg atatggcctc gctccctccg actacatcag
     6721 gctggaagtg acatccatca ttcatcccag cagagcatat gggctcgctg ggaccacagc
     6781 atgcctcgaa gtgctgagag actatcatca aaccgacaga ggatatggcc tcgtcggctc
     6841 ctactccctc ctggtggaat acttctccac tcttcaaccc agcagagcat atgggctcgc
     6901 tgggtccatc agagatacaa gatctctcga gccgcggtgc gtaagtctgt taacagaatg
     6961 ctgcatacgc tgagtacttc gaaatgtcat cctctgtctg acactgaacg taatccagac
     7021 gcggatccta cctacaaacg gcacgagcat cagccgtgcc gcccagcatc ttcatcactg
     7081 tccaatcttc aagcgtgcac gatcacggca cgctccactg tcattacagt cttatccaat
     7141 caaactccag cagcatcagc gctggagctc cgactacatc aggacatcca tcattcaacg
     7201 ctcgacgatc acgcgagcgt ccacagcatg cctcagacac tatcatcaaa cggctgcacg
     7261 atcacggcag ccgctcctac tgcctctacg ttatcgactc ttctgcgacg agcatcagcc
     7321 gtcgcaacat cggacatacc agatcctacc tacaaacggc acgagcatca gccgtgccgc
     7381 ccagcatctt catcactgtc caatcttcaa gcgtgcacga tcacggcacg ctccactgtc
     7441 attacagtct tatccaatca aactccagca gcatcagcgc tggagctccg actacatcag
     7501 gacatccatc attcaacgct cgacgatcac gcgagcgtcc acagcatgcc tcagacacta
     7561 tcatcaaacg gctgcacgat cacggcagcc gctcctactg cctctacgtt atcgactctt
     7621 ctgcgacgag catcagccgt cgcaacatcg gacataccag atcctaccta caaacggcac
     7681 gagcatcagc cgtgccgccc agcatcttca tcactgtcca atcttcaagc gtgcacgatc
     7741 acggcacgct ccactgtcat tacagtctta tccaatcaaa ctccagcagc atcagcgctg
     7801 gagctccgac tacatcagga catccatcat tcaacgctcg acgatcacgc gagcgtccac
     7861 agcatgcctc agacactatc atcaaacggc tgcacgatca cggcagccgc tcctactgcc
     7921 tctacgttat cgactcttct gcgacgagca tcagccgtcg caacatcgga cataccagat
     7981 cctacctaca aacggcacga gcatcagccg tgccgcccag catcttcatc actgtccaat
     8041 cttcaagcgt gcacgatcac ggcacgctcc actgtcatta cagtcttatc caatcaaact
     8101 ccagcagcat cagcgctgga gctccgacta catcaggaca tccatcattc aacgctcgac
     8161 gatcacgcga gcgtccacag catgcctcag acactatcat caaacggctg cacgatcacg
     8221 gcagccgctc ctactgcctc tacgttatcg actcttctgc gacgagcatc agccgtcgca
     8281 acatcggaca taccagatct tcaatcgatt tcgcgcggga tccagaccac ctcccctgcg
     8341 agctaagctg gacagccaat gacgggtaag agagtgacat ttttcactaa cctaagacag
     8401 gagggccgtc agagctactg cctaatccaa agacgggtaa aagtgataaa aatgtatcac
     8461 tccaacctaa gacaggcgca gcttccgagg gatttgagat ccagacatga taagatacat
     8521 tgatgagttt ggacaaacca aaactagaat gcagtgaaaa aaatgcctta tttgtgaaat
     8581 ttgtgatgct attgccttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8641 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     8701 gtaaaacctc tacaaatgtg gtatggctga ttatgatctg aagttcctat actatttgaa
     8761 gaataggaac ttcggaatag gaacttcgga tctcatatgg ccatatgggg gcgcctagag
     8821 aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg
     8881 ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga
     8941 cccagggagg ggggtcaaaa gccaaggctt cccaggtcac gatgtacccc acctggtctg
     9001 ggtgtccatg cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt
     9061 taaaggtgcc gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg
     9121 taacccaacg tctcttcttg acatctaccg actggttgtg agcgatccgc tcgacatctt
     9181 tccagtgacc taaggtcaaa cttaagggag tggtaacagg ctagcccggg cccatatttt
     9241 cagacaaata cagaaacaca gtcagacaga gacaacacag aacgatgctg cagcagacaa
     9301 gacgcgcggc gcggcttcgg tcccaaaccg aaagcaaaaa ttcagacgga ggcgggaact
     9361 gttttaggtt ctcgtctcct accagaacca catatccctc ctctaagggg ggtgcaccaa
     9421 agagtcctaa acgatcggga ttttaggact caggtcgggc cacaaaaacg gcccccgaag
     9481 tccctgggac gtctcccagg gttgcggccg ggtgttccga actcgtcagt tccaccacgg
     9541 gtccgccaga tacagagcta gttagctaac tagtaccgac gcaggcgcat aaaatcagtc
     9601 atagacacta gacaatcgga cagacacaga taagttgctg gccagcttac ctcccggtgg
     9661 tgggtcggtg gtccctgggc aggggtctcc cgatcccgga cgagccccca aatgaaagac
     9721 ccccgctgac gggtagtcaa tcactcagag gagaccctcc caaggaacag cgagaccaca
     9781 agtcggatgc aactgcaaga gggtttattg gatacacggg tacccgggcg actcagtcaa
     9841 tcggaggact ggcgccccga gtgaggggtt gtgggctctt ttattgagct cggggagcat
     9901 aagcgcgcga acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc
     9961 atttcaggtc cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc
    10021 tggaccgcat ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca
    10081 cagatatcct gtttggccca tattcagctg ttccatctgt tcttggccct gagccggggc
    10141 aggaactgct taccacagat atcctgtttg gcccatattc agctgttcca tctgttcctg
    10201 accttgatct gaacttttct attctcagtt atgtattttt ccatgccttg caaaatggcg
    10261 ttacttaagc tagcttgcca aacctacagg tggggtcttt cattcacatg tatatgtcaa
    10321 aaataaaaat caactaattg actagtaatt aatatgactg gcataatggg aaattgatcc
    10381 tgacagatgc aaactggctt gctcagcagc gcatttatgt tgtcaactga ggaaggaaac
    10441 gttaatgaca gaaactctaa gtaatttcca cgtttatcta tttttattta tactagcttt
    10501 ggtaacagga atattgcagc attcatgcac attgaaaccc ttatgaaata aaaacatctg
    10561 tgcatttaaa atggaattaa cattttaaat gttaaaaaaa gctggcttag cttccccccg
    10621 ccccctaggg catagaacaa gtcaaatgct ttatatattt gagtttggga tgtattagga
    10681 aactcctaag agcaaagcta gcttg
//
