LOCUS       sequence-163443-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used as a donor for subcloning into Gateway 
            destination vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pDONR221-rap1-K462-463-651R
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata
       61 atgccaactt tgtacaaaaa agcaggctac aaaatgtcta gtccagatga ttttgaaact
      121 gcaccagcag aatatgttga tgcattggat ccgagtatgg tcgttgttga cagtggttct
      181 gctgcagtca cagcgccatc ggattcagcc gctgaggtaa aagctaatca aaacgaagaa
      241 aacactggtg ctactgctgc tgaaactagt gagaaagtgg atcagacgga ggtcgaaaag
      301 aaagatgatg atgataccac tgaagtcggt gtaacaacta ccacgccttc cattgctgat
      361 actgctgcta ccgctaacat tgcttcgaca tcaggtgcat ctgttactga acctactact
      421 gatgacaccg ctgctgatga gaaaaaggaa caagtaagtg gtcctcctct gtcaaatatg
      481 aaattctatc ttaatcgcga cgcggatgcg catgactctt taaatgatat tgatcaatta
      541 gcgcgtctga ttagagcaaa cggtggtgaa gtgctagact ccaagccaag agaatcaaaa
      601 gagaatgttt tcatcgtgtc accctataat cataccaatt taccaacggt aactccgact
      661 tatattaagg catgttgtca aagcaactcg ctactgaaca tggaaaatta tttagtacca
      721 tacgataact ttagagaagt tgtcgatagt aggttgcagg aggaatcgca ttctaatggt
      781 gtagataata gcaatagcaa tagcgataac aaggattcta tcaggcccaa aacagaaata
      841 atctctacga atactaatgg cgctacggaa gactcaacca gcgaaaaagt tatggtagac
      901 gctgaacaac aagctagatt acaagagcaa gctcagctcc ttcgtcaaca tgtaagcagc
      961 accgcatcaa tcacaagcgg agggcacaat gacttggtcc agatcgaaca acctcaaa
//
