LOCUS       sequence-163444-        8675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human histone deacetylase 4 with flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Fischle W, Emiliani S, Hendzel MJ, Nagase T, Nomura N, Voelter W, 
            Verdin E
  TITLE     A new family of human histone deacetylases related to Saccharomyces 
            cerevisiae HDA1p.
  JOURNAL   J Biol Chem. 1999 Apr 23. 274(17):11713-20.
  PUBMED    10206986
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Apr 23. 274(17):11713-20."
COMMENT     Sequence Label: HDAC4 Flag
FEATURES             Location/Qualifiers
     source          1..8675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3265..3288
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3323..3340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3329..3553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3599..4027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3686..3705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3896..3917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4036..4056)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4041..4370
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4221..4356
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4283..4302
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4437..5231
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4491..4510)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5101..5120
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5405..5526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5442..5461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5496..5515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5575..5591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5575..5591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5588..5610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5599..5615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5623..5629,5630..5647,5648..5653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5668..5689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5806..5823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5977..6565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6057..6076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6736..7527,7528..7596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7359..7378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7597..7701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7776..7795)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7967..8346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8347..8550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8501..8521
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8595..8614
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8595..8613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgagctccc aaagccatcc agatggactt tctggccgag accagccagt ggagctgctg
       61 aatcctgccc gcgtgaacca catgcccagc acggtggatg tggccacggc gctgcctctg
      121 caagtggccc cctcggcagt gcccatggac ctgcgcctgg accaccagtt ctcactgcct
      181 gtggcagagc cggccctgcg ggagcagcag ctgcagcagg agctcctggc gctcaagcag
      241 aagcagcaga tccagaggca gatcctcatc gctgagttcc agaggcagca cgagcagctc
      301 tcccggcagc acgaggcgca gctccacgag cacatcaagc aacaacagga gatgctggcc
      361 atgaagcacc agcaggagct gctggaacac cagcggaagc tggagaggca ccgccaggag
      421 caggagctgg agaagcagca ccgggagcag aagctgcagc agctcaagaa caaggagaag
      481 ggcaaagaga gtgccgtggc cagcacagaa gtgaagatga agttacaaga atttgtcctc
      541 aataaaaaga aggcgctggc ccaccggaat ctgaaccact gcatttccag cgaccctcgc
      601 tactggtacg ggaaaacgca gcacagttcc cttgaccaga gttctccacc ccagagcgga
      661 gtgtcgacct cctataacca cccggtcctg ggaatgtacg acgccaaaga tgacttccct
      721 cttaggaaaa cagcttctga accgaatctg aaattacggt ccaggctaaa gcagaaagtg
      781 gccgaaagac ggagcagccc cctgttacgc aggaaagacg ggccagtggt cactgctcta
      841 aaaaagcgtc cgttggatgt cacagactcc gcgtgcagca gcgccccagg ctccggaccc
      901 agctcaccca acaacagctc cgggagcgtc agcgcggaga acggtatcgc gcccgccgtc
      961 cccagcatcc cggcggagac gagtttggcg cacagacttg tggcacgaga aggctcggcc
     1021 gctccacttc ccctctacac atcgccatcc ttgcccaaca tcacgctggg cctgcctgcc
     1081 accggcccct ctgcgggcac ggcgggccag caggacaccg agagactcac ccttcccgcc
     1141 ctccagcaga ggctctccct tttccccggc acccacctca ctccctacct gagcacctcg
     1201 cccttggagc gggacggagg ggcagcgcac agccctcttc tgcagcacat ggtcttactg
     1261 gagcagccac cggcacaagc acccctcgtc acaggcctgg gagcactgcc cctccacgca
     1321 cagtccttgg ttggtgcaga ccgggtgtcc ccctccatcc acaagctgcg gcagcaccgc
     1381 ccactggggc ggacccagtc ggccccgctg ccccagaacg cccaggctct gcagcacctg
     1441 gtcatccagc agcagcatca gcagtttctg gagaaacaca agcagcagtt ccagcagcag
     1501 caactgcaga tgaacaagat catccccaag ccaagcgagc cagcccggca gccggagagc
     1561 cacccggagg agacggagga ggagctccgt gagcaccagg ctctgctgga cgagccctac
     1621 ctggaccggc tgccggggca gaaggaggcg cacgcacagg ccggcgtgca ggtgaagcag
     1681 gagcccattg agagcgatga ggaagaggca gagcccccac gggaggtgga gccgggccag
     1741 cgccagccca gtgagcagga gctgctcttc agacagcaag ccctcctgct ggagcagcag
     1801 cggatccacc agctgaggaa ctaccaggcg tccatggagg ccgccggcat ccccgtgtcc
     1861 ttcggcggcc acaggcctct gtcccgggcg cagtcctcac ccgcgtctgc caccttcccc
     1921 gtgtctgtgc aggagccccc caccaagccg aggttcacga caggcctcgt gtatgacacg
     1981 ctgatgctga agcaccagtg cacctgcggg agtagcagca gccaccccga gcacgccggg
     2041 aggatccaga gcatctggtc ccgcctgcag gagacgggcc tccggggcaa atgcgagtgc
     2101 atccgcggac gcaaggccac cctggaggag ctacagacgg tgcactcgga agcccacacc
     2161 ctcctgtatg gcacgaaccc cctcaaccgg cagaaactgg acagtaagaa acttctaggc
     2221 tcgctcgcct ccgtgttcgt ccggctccct tgcggtggtg ttggggtgga cagtgacacc
     2281 atatggaacg aggtgcactc ggcgggggca gcccgcctgg ctgtgggctg cgtggtagag
     2341 ctggtcttca aggtggccac aggggagctg aagaatggct ttgctgtggt ccgcccccct
     2401 ggacaccatg cggaggagag cacgcccatg ggcttttgct acttcaactc cgtggccgtg
     2461 gcagccaagc ttctgcagca gaggttgagc gtgagcaaga tcctcatcgt ggactgggac
     2521 gtgcaccatg gaaacgggac ccagcaggct ttctacagcg accctagcgt cctgtacatg
     2581 tccctccacc gctacgacga tgggaacttc ttcccaggca gcggggctcc tgatgaggtg
     2641 ggcacagggc ccggcgtggg tttcaacgtc aacatggctt tcaccggcgg cctggacccc
     2701 cccatgggag acgctgagta cttggcggcc ttcagaacgg tggtcatgcc gatcgccagc
     2761 gagtttgccc cggatgtggt gctggtgtca tcaggcttcg atgccgtgga gggccacccc
     2821 acccctcttg ggggctacaa cctctccgcc agatgcttcg ggtacctgac gaagcagctg
     2881 atgggcctgg ctggcggccg gattgtcctg gccctcgagg gaggccacga cctgaccgcc
     2941 atttgcgacg cctcggaagc atgtgtttct gccttgctgg gaaacgagct tgatcctctc
     3001 ccagaaaagg ttttacagca aagacccaat gcaaacgctg tccgttccat ggagaaagtc
     3061 atggagatcc acagcaagta ctggcgctgc ctgcagcgca caacctccac agcggggcgt
     3121 tctctgatcg aggctcagac ttgcgagaac gaagaagccg agacggtcac cgccatggcc
     3181 tcgctgtccg tgggcgtgaa gcccgccgaa aagagaccag atgaggagcc catggaagag
     3241 gagccgcccc tggaattcgg aggagactac aaggacgacg atgacaagta atctagaggg
     3301 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3361 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3421 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3481 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3541 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3601 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3661 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3721 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3781 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3841 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3901 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3961 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4021 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4081 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4141 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4201 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4261 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4321 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4381 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     4441 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4501 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4561 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     4621 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4681 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4741 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4801 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4861 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4921 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     4981 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5041 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5101 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5161 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5221 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5281 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5341 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5401 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5521 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5581 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5641 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5701 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5761 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5821 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5881 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6721 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6781 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6841 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6901 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6961 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7021 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7081 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7141 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7201 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7261 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7321 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7381 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7441 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7501 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7561 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7621 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7681 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     7741 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7801 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     7861 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     7921 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     7981 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     8041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     8101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     8161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     8221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     8281 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     8341 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     8401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     8461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     8521 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     8581 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
     8641 ttaagcttgg taccgagctc ggatccgaat tcacc
//
