LOCUS       sequence-163445-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used as a donor for subcloning into Gateway 
            destination vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pDONR221-rap1-K462-463-651R
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gttcataaca
      121 ggtccttctc aaaaaatctt ttcctcattt ccattctacc agttccatgc tttttaacca
      181 gtttccttat ttgttcttga tcattgcttt ttagcgattc gtcgtcatca tgtgtccaaa
      241 taccaggaac gttgggagga ggattaacat tgtccttaaa catgttcaag aaatagcgag
      301 gaaagaccat cagatccccg gataaacacg tcaatatgct cgtactgaaa tttttacgta
      361 taccagtttc atcgcaaaga tcctgtacca gtttttcagc ctgtgatggc tcataatctc
      421 ctgaaataac ttcatgaagt ttatccataa attgtctcgt actgccaaaa tgaaagaaca
      481 atggcaggaa aagatctgtt gttgcaattt ttggtggaaa gcttatggta tcaggatcaa
      541 tattatcata tatatcttca ttcgaaaaat cgcttcttat agcttccgca atctcgtgtg
      601 gatactcaaa gggcaaacta tttgaaatat tggatagatc atttcttaaa ctggaaatga
      661 aattcatggt atcttcgtcc agtaattcgt tttctggtat agcgtaagag ttggaagcag
      721 cggcagcagc agcggcagca gcattagcgg acgctgcatt ggcagtaggc tgaacatttc
      781 tttgagaact ataatttctt gccctcttgg cggcagagtt ataattgcca ggagtgggaa
      841 cgccaggcct cttgggtcta ttggtgaggt tcttcatcgg ttccggctct ctattctgag
      901 ctttttcagc ttcataa
//
