LOCUS       sequence-163449-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based hedgehog signal reporter plasmid for zebrafish 
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Mich JK, Payumo AY, Rack PG, Chen JK
  TITLE     In vivo imaging of Hedgehog pathway activation with a nuclear 
            fluorescent reporter.
  JOURNAL   PLoS One. 2014 Jul 28;9(7):e103661. doi: 
            10.1371/journal.pone.0103661. eCollection 2014.
  PUBMED    25068273
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-28"; volume: "9(7):e103661. doi"; pages: " 
            10.1371/journal.pone.0103661. eCollection 2014"
COMMENT     Sequence Label: 8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             258..278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             282..302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             306..326
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    556..690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(580..599)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     634..653
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(812..949,950..961)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(991..1010)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(992..1010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
       61 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      121 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      181 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      241 agctgtacaa gtccgatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg
      301 tagatccaaa aaagaagaga aaggtagggt acaagaagct tagccatggc ttcccgccgg
      361 cggtggcggc gcaggatgat ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg
      421 accgtcaccc tgcagcctgt gcttctgcta ggatcaatgt gtgataggaa ttcctgcaga
      481 agcttgatat cgatggatcc tgtacaagta aagcggccgc caccgcggtg gagctcgaat
      541 taattcatcg atgatgatcc agacatgata agatacattg atgagtttgg acaaaccaca
      601 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      661 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
      721 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
      781 atggctgatt atgatcctct agatcagatc taatactcaa gtacaatttt aatggagtac
      841 ttttttactt ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa
      901 ttttccctaa gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct
      961 gtcaagaacc atatgccggt acccaattcg ccctatagtg agtcgtatta c
//
