LOCUS       sequence-163455-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based hedgehog signal reporter plasmid for zebrafish 
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Mich JK, Payumo AY, Rack PG, Chen JK
  TITLE     In vivo imaging of Hedgehog pathway activation with a nuclear 
            fluorescent reporter.
  JOURNAL   PLoS One. 2014 Jul 28;9(7):e103661. doi: 
            10.1371/journal.pone.0103661. eCollection 2014.
  PUBMED    25068273
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-28"; volume: "9(7):e103661. doi"; pages: " 
            10.1371/journal.pone.0103661. eCollection 2014"
COMMENT     Sequence Label: 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             253..273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             277..297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             301..321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    428..562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(452..471)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     506..525
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(684..821,822..833)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(863..882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(864..882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(892..909)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(892..908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(901..923)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
       61 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      121 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      181 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      241 tacaagtccg atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat
      301 ccaaaaaaga agagaaaggt agggtacaag aagtgatagg aattcctgca gaagcttgat
      361 atcgatggat cctgtacaag taaagcggcc gccaccgcgg tggagctcga attaattcat
      421 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      481 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      541 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      601 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
      661 ttatgatcct ctagatcaga tctaatactc aagtacaatt ttaatggagt acttttttac
      721 ttttactcaa gtaagattct agccagatac ttttactttt aattgagtaa aattttccct
      781 aagtacttgt actttcactt gagtaaaatt tttgagtact ttttacacct ctgtcaagaa
      841 ccatatgccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
      901 cgttttacaa cgtcgtgact ggg
//
