LOCUS       sequence-163456-         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based hedgehog signal reporter plasmid for zebrafish 
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Mich JK, Payumo AY, Rack PG, Chen JK
  TITLE     In vivo imaging of Hedgehog pathway activation with a nuclear 
            fluorescent reporter.
  JOURNAL   PLoS One. 2014 Jul 28;9(7):e103661. doi: 
            10.1371/journal.pone.0103661. eCollection 2014.
  PUBMED    25068273
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-28"; volume: "9(7):e103661. doi"; pages: " 
            10.1371/journal.pone.0103661. eCollection 2014"
COMMENT     Sequence Label: 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggtccccaa actcaccctg aagttctcag gatcggtcgc tagcttggct gcccgcacag
       61 ccctgctcac cgtctccgca gctcagccct ggctggtcgc tccgtgctct ccggtctcac
      121 agccccgctt ttacgtgccc cggagcctgg gctggccctg caggtcgact ctagaggatc
      181 cccgggaaca gattcgcgat cgagagcttc tacttgggtg ttccctgctt gtcggagctt
      241 ctacttgggt gttccctgct tgtcgaggtc gagagcttct acttgggtgt tccctgcttg
      301 tcggagcttc tacttgggtg ttccctgctt gtcgaggtcg agagcttcta cttgggtgtt
      361 ccctgcttgt cggagcttct acttgggtgt tccctgcttg tcgaggtcga gagcttctac
      421 ttgggtgttc cctgcttgtc ggaacttcta cttgggtgtt ccctgcttgt cgaggtcgat
      481 cgcgaatctg ttcccgggga tccagacatg ataa
//
