LOCUS       sequence-163461-       10040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  microRNA reporter relying on a bidirectional PGK promoter. Contains 
            MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA 
            binding sites. Contains a Hygromycin selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     Sequence Label: miReporter-PGK
FEATURES             Location/Qualifiers
     source          1..10040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(122..190,191..982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(340..359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1153..1741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1642..1661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1941..2440
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             2457..3482
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2457..3482
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    3489..3713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4050..4105
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     intron          4302..4874
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(4936..5643)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5211..5230)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5480..5498
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(5650..6027)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        7200..7699
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7716..8093
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(8100..8102,8103..8105,8106..8816)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8145..8166)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8406..8425)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8753..8774
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          8878..9450
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    9647..9702
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 aattcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
       61 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      181 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      241 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      301 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      361 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      421 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      481 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      541 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      601 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      661 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      721 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      781 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      841 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      901 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
      961 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1021 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1081 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1261 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1741 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcc atgatatcaa
     1921 acgcgtaact cgagtctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     1981 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     2041 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     2101 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     2161 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     2221 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     2281 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     2341 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     2401 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acgtcgagtc gccaccatga
     2461 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     2521 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     2581 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     2641 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     2701 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     2761 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     2821 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     2881 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     2941 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     3001 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     3061 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     3121 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     3181 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     3241 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     3301 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     3361 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     3421 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     3481 aggtcgagct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     3541 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     3601 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     3661 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggtcgacgc
     3721 agtcgagttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     3781 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     3841 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     3901 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     3961 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     4021 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     4081 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     4141 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
     4201 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttctttgcca
     4261 aaatgatgag acagcacaac aaccagcacg ttgcccagga gctgtaggaa agagaagaag
     4321 gcatgaacat ggttagcaga ggggcccggt ttggactcag agtattttat cctcatctca
     4381 aacagtgtat atcattgtaa ccataaagag aaaggcagga tgatgaccag ggtgtagttg
     4441 tttctaccaa taagaatatt tccacgccag ccagaattta tatgcagaaa tattctacct
     4501 tatcatttaa ttataacaat tgttctctaa aactgtgctg aagtacaata taatataccc
     4561 tgattgcctt gaaaaaaaag agattagaga aagtacttac aatctgacaa ataaacaaaa
     4621 gtgaatttaa aaattcgtta caaatgcaag ctaaagttta acgaaaaagt tacagaaaat
     4681 gaaaagaaaa taagaggaga caatggttgt caacagagta gaaagtgaaa gaaacaaaat
     4741 tatcatgagg gtccatggtg atacaaggga catcttccca ttctaaacaa caccctgaaa
     4801 actttgcccc ctccatataa catgaatttt acaatagcga aaaagaaaga acaatcaagg
     4861 gtccccaaac tcaccctgaa gttctcagtc tagctcgact gatcaagatc ttctagactc
     4921 gactcaatct gtagacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     4981 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     5041 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     5101 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     5161 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     5221 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     5281 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     5341 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg gaatgtcggc
     5401 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     5461 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     5521 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     5581 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     5641 catctcgacc ttagcgctgg tgtacttggt gatggcctta gtaccctcgg acacggcgtg
     5701 cttggccaac tccccaggca gcagcaggcg cacggccgtc tggatctccc tggaggtgat
     5761 ggtcgagcgc ttgttgtaat gcgccaggcg ggaagcctca cctgcgatgc gctcgaaaat
     5821 gtcgttcaca aacgaattca tgatgcccat ggccttggac gaaatgccgg tgtcagggtg
     5881 gacctgcttc agaaccttgt acacatagat ggaatagctc tccttgcggc tgcgcttgcg
     5941 cttcttgccg cctttcttct gcgccttagt caccgccttc ttggagccct ttttcggggc
     6001 gggagcagac ttcgctggct ctggcatggt ggcgactcga cgtcgaaagg cccggagatg
     6061 aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc
     6121 cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc
     6181 caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc
     6241 cccaaagctc gagttacgcg taactcgagt ctaccgggta ggggaggcgc ttttcccaag
     6301 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     6361 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     6421 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     6481 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     6541 cagcaccgct gagcaatgga agcgggtagg cgtcgagtct accgggtagg ggaggcgctt
     6601 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     6661 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     6721 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     6781 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     6841 cagatggaca gcaccgctga gcaatggaag cgggtaggcg tcgagtctac cgggtagggg
     6901 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     6961 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     7021 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     7081 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     7141 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcgtc gagtctaccg
     7201 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     7261 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     7321 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     7381 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     7441 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     7501 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     7561 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     7621 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     7681 ctcatctccg ggcctttcga cgtcgagtcg ccaccatgcc agagccagcg aagtctgctc
     7741 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
     7801 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
     7861 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg
     7921 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
     7981 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
     8041 agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct aaggtcgaga
     8101 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     8161 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     8221 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     8281 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
     8341 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     8401 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     8461 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     8521 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     8581 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     8641 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     8701 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     8761 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagacag
     8821 atccatgagt cgaggctagc agatctggat ccgtcgagct agactgagaa cttcagggtg
     8881 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
     8941 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
     9001 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
     9061 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
     9121 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc tctttttttt
     9181 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
     9241 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     9301 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
     9361 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     9421 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     9481 tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     9541 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     9601 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     9661 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     9721 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     9781 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     9841 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     9901 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     9961 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
    10021 tgaactcgac tgcgtcgagc
//
