LOCUS       sequence-163467-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-Epn1 (18-157) E42R  Dr in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub27-Epn1 (18-157)  E42R Dr
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             80..91
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             500..523
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atcagcagga
       61 cgcactgacc gaattcatta aagaggagaa aacgcgtatg agtaaagtag acatggtttg
      121 gatagtcgga ggcagttctg tttaccagga agccatgaat caaccaggcc acctcagact
      181 ctttgtgaca aggatcatgc aggaatttga aagtgacacg tttttcccag aaattgattt
      241 ggggaaatat aaacttctcc cagaataccc aggcgtcctc tctgaggtcc aggaggaaaa
      301 aggcatcaag tataagtttg aagtctacga gaagaaagac aggcgcgccg gcggctcagc
      361 gggctcggga agcggagcgg gatctggcag cggcggtagc gcaggcagca gcgggtcaag
      421 cggtgcctcg agcggcaggc gcgccctgca gatgggcagc agccatcacc atcatcaccc
      481 catggggatc gatacgaccc tggaagttct gttccagggg cccccgcgga tgtcagaggc
      541 ggagataaag gtaagggaag caacttctaa tgatccatgg ggtccctcaa gttctctgat
      601 gtcacgtatc gctgacctga catacaatgt agtggccttc tcagagatta tgagcatggt
      661 ctggaagaga ctcaacgacc atgggaaaaa ctggagacat gtatataaag ctatgacact
      721 gatggagtat ctgataaaga cgggttcaga gcgggtagca cagcaatgca gggaaaacat
      781 ctacgccgtt caaacgctta aagacttcca gtacatagac agagacggca aagaccaggg
      841 tgtgaatgta cgggaaaagg ccaagcaact ggtgactctg ctaaaagacg aggagaggct
      901 acgagaggag aggatccatg cactcaagac caaagagaag atggcacaga cctgaactag
      961 ttaa
//
