LOCUS       sequence-163475-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-Epn1 (18-157) Y83A Dr in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub29-Epn1 (18-157) Y83A Dr
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             78..89
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             498..521
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat cagcaggacg
       61 cactgaccga attcattaaa gaggagaaaa cgcgtatgag taaagtagac atggtttgga
      121 tagtcggagg cagttctgtt taccaggaag ccatgaatca accaggccac ctcagactct
      181 ttgtgacaag gatcatgcag gaatttgaaa gtgacacgtt tttcccagaa attgatttgg
      241 ggaaatataa acttctccca gaatacccag gcgtcctctc tgaggtccag gaggaaaaag
      301 gcatcaagta taagtttgaa gtctacgaga agaaagacag gcgcgccggc ggctcagcgg
      361 gctcgggaag cggagcggga tctggcagcg gcggtagcgc aggcagcagc gggtcaagcg
      421 gtgcctcgag cggcaggcgc gccctgcaga tgggcagcag ccatcaccat catcacccca
      481 tggggatcga tacgaccctg gaagttctgt tccaggggcc cccgcggatg tcagaggcgg
      541 agataaaggt aagggaagca acttctaatg atccatgggg tccctcaagt tctctgatgt
      601 cagagatcgc tgacctgaca tacaatgtag tggccttctc agagattatg agcatggtct
      661 ggaagagact caacgaccat gggaaaaact ggagacatgt atataaagct atgacactga
      721 tggaggcgct gataaagacg ggttcagagc gggtagcaca gcaatgcagg gaaaacatct
      781 acgccgttca aacgcttaaa gacttccagt acatagacag agacggcaaa gaccagggtg
      841 tgaatgtacg ggaaaaggcc aagcaactgg tgactctgct aaaagacgag gagaggctac
      901 gagaggagag gatccatgca ctc
//
