LOCUS       sequence-163476-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-Epn1 (18-157) Y83A Dr in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub29-Epn1 (18-157) Y83A Dr
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(430..453)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggtctgtgcc atcttctctt tggtcttgag tgcatggatc ctctcctctc gtagcctctc
       61 ctcgtctttt agcagagtca ccagttgctt ggccttttcc cgtacattca caccctggtc
      121 tttgccgtct ctgtctatgt actggaagtc tttaagcgtt tgaacggcgt agatgttttc
      181 cctgcattgc tgtgctaccc gctctgaacc cgtctttatc agcgcctcca tcagtgtcat
      241 agctttatat acatgtctcc agtttttccc atggtcgttg agtctcttcc agaccatgct
      301 cataatctct gagaaggcca ctacattgta tgtcaggtca gcgatctctg acatcagaga
      361 acttgaggga ccccatggat cattagaagt tgcttccctt acctttatct ccgcctctga
      421 catccgcggg ggcccctgga acagaacttc cagggtcgta tcgatcccca tggggtgatg
      481 atggtgatgg ctgctgccca tctgcagggc gcgcctgccg ctcgaggcac cgcttgaccc
      541 gctgctgcct gcgctaccgc cgctgccaga tcccgctccg cttcccgagc ccgctgagcc
      601 gccggcgcgc ctgtctttct tctcgtagac ttcaaactta tacttgatgc ctttttcctc
      661 ctggacctca gagaggacgc ctgggtattc tgggagaagt ttatatttcc ccaaatcaat
      721 ttctgggaaa aacgtgtcac tttcaaattc ctgcatgatc cttgtcacaa agagtctgag
      781 gtggcctggt tgattcatgg cttcctgg
//
