LOCUS       sequence-163477-        9780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfCherry2(11) and TagBFP fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pSFFV_sfCherry2(11)_TagBFP
FEATURES             Location/Qualifiers
     source          1..9780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..53
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(123..143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        128..457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      308..443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1591..1656
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1786..1806
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2231..2365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2268..2287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2322..2341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2533..3166
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3213..3338
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3835..4068
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4253..4297
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4582..4699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4852..5259
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5415..6116
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6176..6764
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6229..6249)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6647..6658)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6767..6783)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6768..6784)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6874..7107
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7132..7150)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7133..7150)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7440..7459)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7673..7692)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7792..7814
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7852..7870)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7938..8042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8043..8111,8112..8903)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8261..8280)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9074..9662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9563..9582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      361 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
      421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
      481 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
      541 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
      601 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
      661 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
      721 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
      781 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
      841 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
      901 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
      961 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     1021 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     1081 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     1141 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     1201 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     1261 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     1321 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     1381 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     1441 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     1501 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     1561 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     1621 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     1681 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     1741 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     1801 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     1861 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     1921 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     1981 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     2041 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     2101 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     2161 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     2221 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2341 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     2401 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     2461 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     2521 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     2581 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     2641 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     2701 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     2761 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     2821 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     2881 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     2941 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     3001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     3181 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     3241 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     3301 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     3361 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     3421 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     3481 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3541 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3601 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3661 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     3721 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     3781 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     3841 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     3901 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     3961 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     4021 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     4081 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     4141 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     4201 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     4261 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     4321 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     4381 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     4441 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     4501 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca
     4561 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     4621 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     4681 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggatcga
     4741 taagcttgat atcgaattcc tgcagccccg ataaaataaa agattttatt tagtctccag
     4801 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca
     4861 ttttgcaagg catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt
     4921 acatgaaaat agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg
     4981 gcccggggcc aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag
     5041 atggtcccca gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag
     5101 gctcccccaa ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt
     5161 ctcgcttctg ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca
     5221 ctcggcgcgc cagtcctccg acagactgag tcgcccgggg gggatctgga gctctcgaga
     5281 attctcacgc gtctgcagga tatcaagctt gcggtaccgc gggcccggga tccatgtaca
     5341 ccatcgtgga gcagtacgag agagccgagg ccagacacag caccggcgga gcttcgaatc
     5401 ctgcaccagg aagtatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     5461 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     5521 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     5581 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     5641 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     5701 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     5761 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     5821 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     5881 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
     5941 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     6001 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     6061 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattaagcgg
     6121 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca
     6181 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6241 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6301 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6361 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6421 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6481 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6541 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6601 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6661 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6721 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     6781 tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     6841 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6901 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6961 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7021 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7081 tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg
     7141 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa
     7201 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc
     7261 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa
     7321 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat
     7381 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     7441 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     7501 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     7561 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     7621 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
     7681 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7741 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7801 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7861 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8281 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
//
