LOCUS       sequence-163481-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-GPD1L
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattcccctc tagaaataat tttgtttaaa ctttaagaag gagatataca tatgaaacat
       61 catcatcatc atcaccagat ggcagcggcg cccctgaaag tgtgcatcgt gggctcgggg
      121 aactggggtt cagctgttgc aaaaataatt ggtaataatg tcaagaaact tcagaaattt
      181 gcctccacag tcaagatgtg ggtctttgaa gaaacagtga atggcagaaa actgacagac
      241 atcataaata atgaccatga aaatgtaaaa tatcttcctg gacacaagct gccagaaaat
      301 gtggttgcca tgtcaaatct tagcgaggct gtgcaggatg cagacctgct ggtgtttgtc
      361 attccccacc agttcattca cagaatctgt gatgagatca ctgggagagt gcccaagaaa
      421 gcgctgggaa tcaccctcat caagggcata gacgagggcc ccgaggggct gaagctcatt
      481 tctgacatca tccgtgagaa gatgggtatt gacatcagtg tgctgatggg agccaacatt
      541 gccaatgagg tggctgcaga gaagttctgt gagaccacca tcggcagcaa agtaatggag
      601 aacggccttc tcttcaaaga acttctgcag actccaaatt ttcgaattac cgtggttgat
      661 gatgcagaca ctgttgaact ctgtggtgcg cttaagaaca tcgtagctgt gggagctggg
      721 ttctgcgacg gcctccgctg tggagacaac accaaagcgg ccgtcatccg cctgggactc
      781 atggaaatga ttgcttttgc caggatcttc tgcaaaggcc aagtgtctac agccaccttc
      841 ctagagagct gcggggtggc cgacctgatc accacctgtt acggagggcg gaaccgcagg
      901 gtggccgagg ccttcgccag aactgggaag accattgaag agttggagaa ggagatgctg
      961 aatgggcaaa agctccaagg accgcagact tctgctgaag tgta
//
