LOCUS       sequence-163491-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLentiCRISPRv1-sgPHGDH-G5
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             58..78
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtcgtgacg cgggatccgc caccatggat tacaaagacg atgacgataa gatggcccca
       61 aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc
      121 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
      181 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
      241 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
      301 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
      361 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
      421 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
      481 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
      541 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
      601 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
      661 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
      721 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
      781 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
      841 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
      901 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggc
//
