LOCUS       sequence-163492-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLentiCRISPRv1-sgPHGDH-G5
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..97)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cgccggcctg cttcagcagg ctgaagttgg tggcgccgct gccgctagct ttctttttct
       61 tagcttgacc agctttctta gtagcagcag gacgcttgtc gcctcccagc tgagacaggt
      121 cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct
      181 ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag
      241 gggctcccag attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg
      301 gcttatcccg gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga
      361 tcactctctt ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct
      421 gttccacaaa cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt
      481 ggctggccag gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct
      541 gcagttcgcc ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt
      601 acttaggcag cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa
      661 agtcgatggg attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt
      721 tcacactctt cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat
      781 aggccacggt ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg
      841 cgatcagctt atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct
      901 gcacctcggt ct
//
