LOCUS       sequence-163509-       10432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter 
            for isopentenol production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   George KW, Thompson MG, Kang A, Baidoo E, Wang G, Chan LJ, Adams PD,
            Petzold CJ, Keasling JD, Lee TS
  TITLE     Metabolic engineering for the high-yield production of 
            isoprenoid-based C(5) alcohols in E. coli.
  JOURNAL   Sci Rep. 2015 Jun 8;5:11128. doi: 10.1038/srep11128.
  PUBMED    26052683
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-8"; volume: "5:11128. doi"; pages: " 
            10.1038/srep11128"
COMMENT     Sequence Label: JBEI-6823
FEATURES             Location/Qualifiers
     source          1..10432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(2309..2320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5219..5230
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      8597..8668
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8684..8711
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(8739..8756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8873..9418)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8953..8972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9532..9626)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9647..10306)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10221..10240
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10307..10409)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttaggag gaatataaaa tgaaaaattg
     1621 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
     1681 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
     1741 ttcacaacac gttgatgaag tgattatggg taacgtgtta caagccgggc tggggcaaaa
     1801 tccggcgcgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg gattcacggt
     1861 caataaagta tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg ccattcaggc
     1921 aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag ccccctactt
     1981 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
     2041 cctgcgcgat ggcctgatgt gcgccaccca tggttatcat atggggatta ccgccgaaaa
     2101 cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc tacattcaca
     2161 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
     2221 tgttgtcact cgaaagaaaa ccttcgtctt cagtcaagac gagttcccga aagcgaactc
     2281 aacggctgaa gcgttaggtg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
     2341 tgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg aagaatctgc
     2401 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
     2461 gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt tacaactggc
     2521 ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg ctgcacagtt
     2581 ccttgccgtt gggaaaaacc tgggctttga ttctgagaaa gtgaatgtca acggcggggc
     2641 catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca cactattaca
     2701 tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctgtgcattg gcggcggtca
     2761 gggaattgcg atggtgattg aacggttgaa ttgaggatct aggaggaaag caaaatgaaa
     2821 ctgagcacca agctgtgctg gtgtggcatc aagggtcgcc tgcgcccaca aaagcagcaa
     2881 cagctgcaca acacgaacct gcaaatgacc gagctgaaaa agcagaagac ggccgagcaa
     2941 aagacccgcc cgcagaacgt tggcatcaag ggcatccaga tttatatccc gacgcagtgt
     3001 gtcaaccaat ctgagctgga gaaattcgat ggcgtcagcc agggtaagta caccatcggc
     3061 ctgggccaga ccaacatgag cttcgtgaac gaccgtgagg acatctattc tatgagcctg
     3121 acggtgctgt ctaagctgat caagagctac aacatcgaca cgaataagat cggtcgtctg
     3181 gaggtgggta cggagacgct gattgacaag agcaaaagcg tgaagtctgt cttaatgcag
     3241 ctgttcggcg agaacacgga tgtcgagggt atcgacaccc tgaacgcgtg ttacggcggc
     3301 accaacgcac tgttcaatag cctgaactgg attgagagca acgcctggga tggccgcgat
     3361 gcgatcgtcg tgtgcggcga tatcgccatc tatgacaagg gtgcggcacg tccgaccggc
     3421 ggtgcaggca ccgttgcgat gtggattggc ccggacgcac caattgtctt cgattctgtc
     3481 cgcgcgtctt acatggagca cgcctacgac ttttacaagc cggacttcac gagcgaatac
     3541 ccgtacgtgg acggccactt ctctctgacc tgctatgtga aggcgctgga ccaggtttat
     3601 aagtcttata gcaaaaaggc gatttctaag ggcctggtca gcgacccggc aggcagcgac
     3661 gccctgaacg tgctgaagta tttcgactac aacgtgttcc atgtcccgac ctgcaaatta
     3721 gtgaccaaat cttatggccg cctgttatat aatgatttcc gtgccaaccc gcagctgttc
     3781 ccggaggttg acgccgagct ggcgacgcgt gattacgacg agagcctgac cgacaagaac
     3841 atcgagaaga ccttcgtcaa cgtcgcgaag ccgttccaca aagagcgtgt ggcccaaagc
     3901 ctgatcgtcc cgaccaacac gggcaacatg tataccgcgt ctgtctacgc ggcattcgcg
     3961 agcctgctga attacgtcgg ttctgacgac ctgcagggca agcgcgttgg cctgttcagc
     4021 tacggtagcg gcttagcggc cagcctgtat agctgcaaaa ttgtcggcga cgtccagcac
     4081 atcatcaagg agctggacat caccaacaag ctggcgaagc gcatcaccga gacgccgaaa
     4141 gattacgagg cagcgatcga gttacgcgag aatgcgcatc tgaagaagaa cttcaagccg
     4201 caaggtagca tcgagcacct gcagagcggc gtctactacc tgacgaacat tgacgacaag
     4261 ttccgccgtt cttatgacgt caaaaagtaa ctagtaggag gaaaacatca tggtgctgac
     4321 gaacaaaacc gtcattagcg gcagcaaggt gaagtctctg agcagcgccc aaagctctag
     4381 cagcggcccg tctagcagca gcgaggagga cgacagccgt gacattgagt ctctggacaa
     4441 gaagatccgc ccgctggagg agttagaggc cctgctgagc agcggcaaca ccaagcagct
     4501 gaagaacaag gaagttgcag cgctggtgat ccacggtaag ctgccactgt atgcgctgga
     4561 aaagaaactg ggcgatacga cgcgtgcggt cgcggtgcgt cgcaaagcct taagcatctt
     4621 agcggaggcc ccggtgttag ccagcgaccg cctgccgtac aagaactacg actacgaccg
     4681 cgtgtttggc gcgtgctgcg agaatgtcat tggctacatg ccgttaccgg ttggtgtgat
     4741 cggcccgctg gtcattgatg gcacgagcta tcacattcca atggcgacca cggaaggttg
     4801 cttagtcgcc agcgccatgc gtggctgtaa ggcgattaac gccggcggtg gcgcgacgac
     4861 cgtgttaacc aaggatggta tgacgcgcgg tccggtcgtc cgcttcccaa cgctgaagcg
     4921 cagcggcgcg tgtaagattt ggctggattc tgaggagggc caaaacgcga tcaagaaagc
     4981 cttcaactct acgagccgtt tcgcgcgttt acagcatatc cagacctgcc tggccggcga
     5041 cctgctgttc atgcgcttcc gcaccaccac gggcgatgcg atgggcatga acatgatcag
     5101 caagggcgtc gaatatagcc tgaaacaaat ggtggaagaa tatggctggg aggacatgga
     5161 ggttgtctct gtgagcggca actattgcac cgacaagaag ccggcagcca ttaactggat
     5221 tgagggtcgc ggcaaaagcg tcgtggcaga agcgaccatc ccaggcgacg tggtccgtaa
     5281 ggttctgaag agcgacgtca gcgccctggt tgagttaaat atcgcgaaaa acctggtcgg
     5341 cagcgcgatg gcgggcagcg tgggtggctt taacgcacat gcagcgaatc tggttacggc
     5401 ggttttctta gccttaggtc aggacccagc ccaaaatgtc gagagcagca actgcattac
     5461 cttaatgaaa gaggttgacg gtgacctgcg catcagcgtt tctatgccgt ctatcgaggt
     5521 cggcacgatc ggcggcggca ccgttttaga accgcaaggt gcgatgctgg atctgctggg
     5581 cgtgcgcggc ccacatgcaa cggccccagg caccaatgcc cgccaactgg cccgtatcgt
     5641 ggcctgcgcg gttctggcgg gtgagctgag cctgtgcgcc gcattagccg cgggccattt
     5701 agttcaatct cacatgaccc acaaccgcaa gccggcagaa ccaaccaagc caaataacct
     5761 ggacgcaacc gacattaacc gtctgaagga tggcagcgtc acgtgcatta aaagctgagg
     5821 atctggatct aaaggatcta ggagggagat catatgagcg aattacgtgc attcagcgcg
     5881 ccaggtaagg cactgctggc cggtggctac ctggtgttag acaccaagta cgaggcgttc
     5941 gtcgtcggct tatctgcccg tatgcatgca gttgcccacc cgtatggtag cctgcagggc
     6001 tctgacaagt tcgaagtgcg tgtgaagagc aagcagttca aggacggcga gtggctgtac
     6061 cacattagcc caaagagcgg cttcatcccg gttagcattg gtggcagcaa gaacccattt
     6121 atcgagaagg tcattgccaa cgtcttcagc tacttcaagc cgaatatgga cgattactgc
     6181 aaccgcaacc tgttcgtcat cgacattttc agcgacgacg cgtaccacag ccaagaggac
     6241 tctgttacgg agcatcgtgg taaccgccgc ctgagcttcc acagccatcg cattgaggag
     6301 gtgccgaaga cgggtctggg ttctagcgcc ggtttagtta ccgtcttaac gacggcgtta
     6361 gcgagcttct tcgtgagcga cctggagaac aacgtggaca agtaccgcga agtgattcat
     6421 aacctggcgc aggtggcaca ttgtcaggcc caaggtaaga ttggctctgg ttttgatgtg
     6481 gcagcggccg cctatggctc tatccgctat cgccgctttc cgccggccct gatcagcaat
     6541 ctgccggaca tcggctctgc gacgtatggt agcaaactgg cgcatctggt ggacgaagaa
     6601 gactggaaca tcaccattaa gtctaatcac ctgccgagcg gcttaacgtt atggatgggc
     6661 gatatcaaga acggcagcga aacggttaag ctggtgcaga aagtgaaaaa ctggtacgac
     6721 agccacatgc cggaaagcct gaagatttac acggagctgg accacgccaa tagccgtttc
     6781 atggatggtc tgagcaagct ggaccgcctg cacgaaaccc acgacgacta cagcgaccaa
     6841 atcttcgaga gcctggagcg caatgactgc acctgccaga agtacccgga gatcacggag
     6901 gtccgcgatg ccgtggcaac gattcgccgt agcttccgca aaattacgaa ggagagcggc
     6961 gcggatatcg aaccaccggt ccagacgtct ctgctggacg actgtcaaac cttaaagggc
     7021 gtgttaacgt gcctgattcc gggcgcgggt ggttacgacg ccattgccgt catcacgaaa
     7081 caggacgtcg atctgcgcgc acaaacggcc aacgacaaac gtttcagcaa agtccaatgg
     7141 ctggatgtta cgcaggccga ctggggtgtt cgcaaggaga aggacccgga aacgtatctg
     7201 gataagtgag gatctaggag gaaataacca tgtctctgcc attcctgacg tctgcgccag
     7261 gtaaggtgat catcttcggc gagcactctg cggtgtacaa taagccggcc gtcgccgcct
     7321 ctgtgtctgc gttacgcacc tacctgctga tcagcgaatc ttctgcaccg gacacgatcg
     7381 agctggactt tccggacatc agcttcaacc acaagtggag catcaacgac ttcaacgcga
     7441 tcacggagga ccaggtgaac agccaaaagc tggccaaagc ccagcaagca accgacggtc
     7501 tgtctcagga gctggtgtct ctgctggacc cgctgttagc gcagttaagc gagagcttcc
     7561 attaccacgc cgcgttctgc ttcctgtaca tgttcgtttg cctgtgcccg cacgcaaaga
     7621 acatcaagtt cagcctgaag agcacgctgc cgattggcgc aggcttaggc tctagcgcat
     7681 ctatcagcgt gagcctggcg ctggcgatgg cctatctggg tggcctgatt ggcagcaacg
     7741 acctggagaa actgagcgaa aacgacaagc acatcgtgaa ccagtgggcc tttatcggcg
     7801 agaagtgcat tcatggcacc ccgagcggca ttgacaacgc agttgccacg tatggcaacg
     7861 ccctgctgtt cgagaaagac agccacaacg gcacgatcaa cacgaacaac ttcaagttcc
     7921 tggacgactt cccggcgatc ccgatgattc tgacctacac ccgtatccca cgcagcacca
     7981 aggatttagt cgcccgcgtg cgtgttttag tcaccgaaaa gttcccggag gtgatgaagc
     8041 cgatcctgga cgcgatgggc gagtgcgcgc tgcagggtct ggagatcatg accaagctga
     8101 gcaagtgcaa gggcaccgac gatgaggcgg tggaaaccaa caatgagctg tacgagcagc
     8161 tgctggagct gatccgtatc aatcacggcc tgctggtttc tatcggtgtg tctcacccgg
     8221 gcctggaact gatcaaaaac ctgagcgacg acctgcgcat tggctctacg aaattaacgg
     8281 gtgcaggtgg cggtggctgc tctttaacgc tgctgcgccg tgacattacg caggagcaaa
     8341 tcgacagctt caagaagaag ctgcaggacg acttcagcta cgagacgttc gagacggacc
     8401 tgggcggcac gggctgttgc ctgctgagcg ccaaaaatct gaacaaggac ctgaagatca
     8461 aaagcctggt gttccagctg ttcgaaaaca agacgaccac gaagcagcag atcgacgacc
     8521 tgttactgcc gggtaacacc aatctgccgt ggacgtctta attaggatcc aaactcgagt
     8581 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     8641 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     8701 gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc
     8761 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     8821 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     8881 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     8941 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     9001 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     9061 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     9121 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     9181 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     9241 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     9301 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     9361 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     9421 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
     9481 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc
     9541 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     9601 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     9661 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     9721 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     9781 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     9841 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     9901 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     9961 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
    10021 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
    10081 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
    10141 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
    10201 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
    10261 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
    10321 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
    10381 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccagata tc
//
