LOCUS       sequence-163518-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 with Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR1-Flag Cterm
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        191..209
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     191..208
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     222..241
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      294..303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt
       61 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      121 ggcccacccc cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca
      181 tacacatacg atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg
      241 tgtccactcc caggtccaac tgcacctcgg ttctatcgat tgaattcgtc gacgccacca
      301 tggagctgca catcctagaa caccgggtgc gggtgctgag cgtcgcccgt cccggtctct
      361 ggctctacac ccacccgctc atcaagctgc tcttcctgcc ccgccgcagc cggtgcaagt
      421 tcttcagcct gacggagacc cctgaggatt acacgcttat ggtggacgag gagggcttta
      481 aagagctgcc cccatctgag ttcctgcaag tagctgaggc cacatggctg gtgctgaacg
      541 tgtcgtctca cagcggtgcg gcagtgcagg ctgctggggt caccaagatc gcccgttcgg
      601 tcatcgcgcc actggccgag caccacgtgt ctgtgctgat gctgtccact taccagacgg
      661 acttcatcct ggtgcgggag caggacctgt ccgtggtgat ccacacgctg gcccaggagt
      721 tcgacattta ccgcgaggtg ggcggagagc ctgtgcctgt gacgagggat gattccagca
      781 atggctttcc ccgcactcag catgggccca gccccacggt gcatcccatc cagagcccac
      841 agaaccgctt ctgtgtcctc acactggacc ctgagacgct tccagccatc gccaccaccc
      901 tcatagatgt cctcttctac tcgcacagca cccccaagga ggcagcctct agcagtcctg
      961 aacccagctc catcacgttc tttgccttct ctctcatcga gggttatatc tccattgtca
     1021 tggatgctga aacacagaaa aagt
//
