LOCUS       sequence-163521-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 with Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR1-Flag Cterm
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(49..72)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcaataaac aagttgggcc atggcggcca agctggggat ccttatcact tgtcatcgtc
       61 atccttgtag tcacctccac ctcctgcggc cgcggaagcc aggccttcct gccgccgctg
      121 gaggacctcg atgacgctgc cgataccgtc ctcgggcacc agggcgtggt cgaagttgaa
      181 ggtgctgatg tagtaggcag agatgtcagc ggcagccagg ggacctgcaa tctgtgccac
      241 gatgccacat tcatcaaagc ccaggggctg tccaccgatg cgcaccatcc tccacagctc
      301 ccccgaggag ctggtcagca ggaggtcact ggggaacttt ttctgtgttt cagcatccat
      361 gacaatggag atataaccct cgatgagaga gaaggcaaag aacgtgatgg agctgggttc
      421 aggactgcta gaggctgcct ccttgggggt gctgtgcgag tagaagagga catctatgag
      481 ggtggtggcg atggctggaa gcgtctcagg gtccagtgtg aggacacaga agcggttctg
      541 tgggctctgg atgggatgca ccgtggggct gggcccatgc tgagtgcggg gaaagccatt
      601 gctggaatca tccctcgtca caggcacagg ctctccgccc acctcgcggt aaatgtcgaa
      661 ctcctgggcc agcgtgtgga tcaccacgga caggtcctgc tcccgcacca ggatgaagtc
      721 cgtctggtaa gtggacagca tcagcacaga cacgtggtgc tcggccagtg gcgcgatgac
      781 cgaacgggcg atcttggtga ccccagcagc ctgcactgcc gcaccgctgt gagacgacac
      841 gttcagcacc agccatgtgg cctcagctac ttgcaggaac tcagatgggg gcagctcttt
      901 aaagccctcc tcgtccacca taagcgtgta atcctcaggg gtctccgtca ggctgaagaa
      961 cttgcaccgg ctgcggcggg gcaggaagag cagcttgatg agcgggtggg
//
