LOCUS       sequence-163526-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR2-HA Cterm
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        192..210
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     192..209
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     223..242
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg
       61 tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat
      121 aggcccaccc ccttggcttc gttagaacgc ggctacaatt aatacataac cttatgtatc
      181 atacacatac gatttaggtg acactataga ataacatcca ctttgccttt ctctccacag
      241 gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttgaattcgt cgacgccacc
      301 atggaactcc acatcctgga gcaccggctg caagttgcca gcgtcgccaa ggagagtatc
      361 ccgctgttca cctacggcct gatcaaactt gccttcctgt cctccaagac caggtgcaag
      421 ttcttcagtc tgactgagac gccagaggat tacactatca ttgtcgatga ggaaggattc
      481 ctagagctgc cctcctcgga gcacctgagt gtggcagatg ccacctggct ggccctgaac
      541 gtggtgtccg gcggtggcag cttctccagc tcccagccca tcggcgtgac caagatcgcc
      601 aagtcagtca tcgccccact ggctgaccag aacatatccg tgttcatgct gtccacgtat
      661 cagacagact tcatcctggt gcgcgagcgg gacctgccct ttgtcaccca cacattgtca
      721 tcagagttca ccatcctgcg ggtcgtcaat ggcgagaccg tggcagccga gaacctcggc
      781 atcaccaatg gcttcgtgaa gcccaagctg gtccagaggc cagtcatcca cccactgtcc
      841 agcccgagca acaggttctg tgtcaccagc ctggaccctg acacgctgcc tgctgttgcc
      901 acactcctca tggatgtcat gttctactcc aatggagtga aggaccccat ggccactggg
      961 gatgactgcg gccacatccg cttcttctcc ttctccctca tcgagggcta catctccctg
     1021 gtgatggacg tgca
//
