LOCUS       sequence-163529-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR2-HA Cterm
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(49..75)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgcaataaac aagttgggcc atggcggcca agctggggat ccttatcatg cgtagtcagg
       61 cacatcatag gggtaacctc cacctcctgc ggccgcgtgc ttctctgctt ggctgacctt
      121 cagggcactg atgacaccat tgatgttctc ttcggggaca agtgcatgat caaacttgaa
      181 agtactgatg tagtaggctg ggatgtctgc agcagccaag ggctctgaga tctgggccac
      241 gatgccacac tcatcaaacc ccaggggctg tcctccaatc cggaccatct tccagagctc
      301 tccggatgcg cttgtgaaca gcaagttact aggaaacctc tgctgcgtct gcacgtccat
      361 caccagggag atgtagccct cgatgaggga gaaggagaag aagcggatgt ggccgcagtc
      421 atccccagtg gccatggggt ccttcactcc attggagtag aacatgacat ccatgaggag
      481 tgtggcaaca gcaggcagcg tgtcagggtc caggctggtg acacagaacc tgttgctcgg
      541 gctggacagt gggtggatga ctggcctctg gaccagcttg ggcttcacga agccattggt
      601 gatgccgagg ttctcggctg ccacggtctc gccattgacg acccgcagga tggtgaactc
      661 tgatgacaat gtgtgggtga caaagggcag gtcccgctcg cgcaccagga tgaagtctgt
      721 ctgatacgtg gacagcatga acacggatat gttctggtca gccagtgggg cgatgactga
      781 cttggcgatc ttggtcacgc cgatgggctg ggagctggag aagctgccac cgccggacac
      841 cacgttcagg gccagccagg tggcatctgc cacactcagg tgctccgagg agggcagctc
      901 taggaatcct tcctcatcga caatgatagt gtaatcctct ggcgtctcag tcagactgaa
      961 gaacttgcac ctggtcttgg aggacaggaa ggcaa
//
