LOCUS       sequence-163532-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 with Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR2-Flag Cterm
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        191..209
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     191..208
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     222..241
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      294..303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt
       61 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      121 ggcccacccc cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca
      181 tacacatacg atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg
      241 tgtccactcc caggtccaac tgcacctcgg ttctatcgat tgaattcgtc gacgccacca
      301 tggaactcca catcctggag caccggctgc aagttgccag cgtcgccaag gagagtatcc
      361 cgctgttcac ctacggcctg atcaaacttg ccttcctgtc ctccaagacc aggtgcaagt
      421 tcttcagtct gactgagacg ccagaggatt acactatcat tgtcgatgag gaaggattcc
      481 tagagctgcc ctcctcggag cacctgagtg tggcagatgc cacctggctg gccctgaacg
      541 tggtgtccgg cggtggcagc ttctccagct cccagcccat cggcgtgacc aagatcgcca
      601 agtcagtcat cgccccactg gctgaccaga acatatccgt gttcatgctg tccacgtatc
      661 agacagactt catcctggtg cgcgagcggg acctgccctt tgtcacccac acattgtcat
      721 cagagttcac catcctgcgg gtcgtcaatg gcgagaccgt ggcagccgag aacctcggca
      781 tcaccaatgg cttcgtgaag cccaagctgg tccagaggcc agtcatccac ccactgtcca
      841 gcccgagcaa caggttctgt gtcaccagcc tggaccctga cacgctgcct gctgttgcca
      901 cactcctcat ggatgtcatg ttctactcca atggagtgaa ggaccccatg gccactgggg
      961 atgactgcgg ccacatccgc ttcttctcct tctccctcat cgagggctac atctccctgg
     1021 tgatggacgt
//
