LOCUS       sequence-163534-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 with Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR2-Flag Cterm
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(51..74)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctgcaataa acaagttggg ccatggcggc caagctgggg atccttatca cttgtcatcg
       61 tcatccttgt agtcacctcc acctcctgcg gccgcgtgct tctctgcttg gctgaccttc
      121 agggcactga tgacaccatt gatgttctct tcggggacaa gtgcatgatc aaacttgaaa
      181 gtactgatgt agtaggctgg gatgtctgca gcagccaagg gctctgagat ctgggccacg
      241 atgccacact catcaaaccc caggggctgt cctccaatcc ggaccatctt ccagagctct
      301 ccggatgcgc ttgtgaacag caagttacta ggaaacctct gctgcgtctg cacgtccatc
      361 accagggaga tgtagccctc gatgagggag aaggagaaga agcggatgtg gccgcagtca
      421 tccccagtgg ccatggggtc cttcactcca ttggagtaga acatgacatc catgaggagt
      481 gtggcaacag caggcagcgt gtcagggtcc aggctggtga cacagaacct gttgctcggg
      541 ctggacagtg ggtggatgac tggcctctgg accagcttgg gcttcacgaa gccattggtg
      601 atgccgaggt tctcggctgc cacggtctcg ccattgacga cccgcaggat ggtgaactct
      661 gatgacaatg tgtgggtgac aaagggcagg tcccgctcgc gcaccaggat gaagtctgtc
      721 tgatacgtgg acagcatgaa cacggatatg ttctggtcag ccagtggggc gatgactgac
      781 ttggcgatct tggtcacgcc gatgggctgg gagctggaga agctgccacc gccggacacc
      841 acgttcaggg ccagccaggt ggcatctgcc acactcaggt gctccgagga gggcagctct
      901 aggaatcctt cctcatcgac aatgatagtg taatcctctg gcgtctcagt cagactgaag
      961 aacttgcacc tggtcttgga ggacaggaag gcaagtttga tcaggccgta ggtgaacagc
     1021 gggatactct ccttgg
//
