LOCUS       sequence-163536-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA cassette-carrying vector with natMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Stovicek V, Borodina I, Forster J
  TITLE     CRISPR-Cas system enables fast and simple genome editing of 
            industrial Saccharomyces cerevisiae strains.
  JOURNAL   Metab Eng Commun. 2015 Mar 20;2:13-22. doi: 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec.
  PUBMED    34150504
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2015-03-20"; volume: "2:13-22. doi"; pages: " 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec"
COMMENT     Sequence Label: pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..66
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        121..464
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gcttcgtacg ctgcaggtcg acaaccctta atataacttc gtataatgta tgctatacga
       61 agttattagg tctagagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc
      121 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
      181 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
      241 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
      301 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
      361 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
      421 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgggt accactcttg
      481 acgacacggc ttaccggtac cgcaccagtg tcccggggga cgccgaggcc atcgaggcac
      541 tggatgggtc cttcaccacc gacaccgtct tccgcgtcac cgccaccggg gacggcttca
      601 ccctgcggga ggtgccggtg gacccgcccc tgaccaaggt gttccccgac gacgaatcgg
      661 acgacgaatc ggacgacggg gaggacggcg acccggactc ccggacgttc gtcgcgtacg
      721 gggacgacgg cgacctggcg ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc
      781 tgaccgtcga ggacatcgag gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt
      841 tgatggggct cgcgacggag ttcgcccgcg agcggggcgc cgggcacctc tggctggagg
      901 tcaccaacgt caacgcaccg gcgatccacg cgtaccggcg gatggggttc accctctgcg
      961 gcctggacac cgccctgtac gacggcaccg cctcggacgg cgagcaggcg ctcta
//
