LOCUS       sequence-163539-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9-carrying vector with kanMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Stovicek V, Borodina I, Forster J
  TITLE     CRISPR-Cas system enables fast and simple genome editing of 
            industrial Saccharomyces cerevisiae strains.
  JOURNAL   Metab Eng Commun. 2015 Mar 20;2:13-22. doi: 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec.
  PUBMED    34150504
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2015-03-20"; volume: "2:13-22. doi"; pages: " 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec"
COMMENT     Sequence Label: pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc
       61 ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg
      121 tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc
      181 ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat
      241 ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc
      301 ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca
      361 cgaacagctg cttctgctca ttatcttcgg gagacccttt gagcttttca tagtggctgg
      421 ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct
      481 cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg
      541 gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga
      601 tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc
      661 ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga
      721 ctgtaggaga atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca
      781 gcttgtcgct gttccttttc gggaggatac tttccttgga gaagcctccg gtctgtactt
      841 cggtcttttt aacgatgttc acctgcggca tggacaggac cttccggact gtcgcgaaat
      901 ccctaccctt gtcccacacg atttctcctg tttctccgtt tgtttcgata agtggtcgct
      961 tccgaatctc tccattggcc a
//
