LOCUS       sequence-163540-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 ACT1 domain (aa 1-169).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-Flag CASTOR1 ACT1
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        196..214
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     196..213
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     227..246
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             305..328
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    909..1043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(946..965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1000..1019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
       61 acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt
      121 ctataggccc acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg
      181 tatcatacac atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc
      241 acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat tcaccatgga
      301 aatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga cgatggagct
      361 gcacatccta gaacaccggg tgcgggtgct gagcgtcgcc cgtcccggtc tctggctcta
      421 cacccacccg ctcatcaagc tgctcttcct gccccgccgc agccggtgca agttcttcag
      481 cctgacggag acccctgagg attacacgct tatggtggac gaggagggct ttaaagagct
      541 gcccccatct gagttcctgc aagtagctga ggccacatgg ctggtgctga acgtgtcgtc
      601 tcacagcggt gcggcagtgc aggctgctgg ggtcaccaag atcgcccgtt cggtcatcgc
      661 gccactggcc gagcaccacg tgtctgtgct gatgctgtcc acttaccaga cggacttcat
      721 cctggtgcgg gagcaggacc tgtccgtggt gatccacacg ctggcccagg agttcgacat
      781 ttaccgcgag gtgggcggag agcctgtgcc tgtgacgagg gatgattcca gcaatggctt
      841 tccccgcact cagcatgggt gagcggccgc taagtaagta aggatcccca gcttggccgc
      901 catggcccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      961 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1021 atgtatctta tcatgtctgg atcgggaatt aattcggcgc a
//
