LOCUS       sequence-163542-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9-carrying vector with kanMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Stovicek V, Borodina I, Forster J
  TITLE     CRISPR-Cas system enables fast and simple genome editing of 
            industrial Saccharomyces cerevisiae strains.
  JOURNAL   Metab Eng Commun. 2015 Mar 20;2:13-22. doi: 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec.
  PUBMED    34150504
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2015-03-20"; volume: "2:13-22. doi"; pages: " 
            10.1016/j.meteno.2015.03.001. eCollection 2015 Dec"
COMMENT     Sequence Label: pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggctcgata tcggcacaaa cagcgtcggt tgggccgtca ttacggacga gtacaaggtg
       61 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
      121 attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca
      181 gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt
      241 aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg
      301 gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg
      361 taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga cagtactgat
      421 aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt tcggggacac
      481 ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa
      541 ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc cggagttgac
      601 gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca
      661 cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct gtcactcggg
      721 ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct tcaactgagc
      781 aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga ccagtacgca
      841 gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga tattctgcga
      901 gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg ctatgatgag
      961 caccaccaag acttgac
//
