LOCUS       sequence-163544-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 ACT1 domain (aa 1-169).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-Flag CASTOR1 ACT1
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(599..622)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(681..700)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(713..731)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(714..731)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(941..965)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(962..1013)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(991..1011)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agctgcaata aacaagttgg gccatggcgg ccaagctggg gatccttact tacttagcgg
       61 ccgctcaccc atgctgagtg cggggaaagc cattgctgga atcatccctc gtcacaggca
      121 caggctctcc gcccacctcg cggtaaatgt cgaactcctg ggccagcgtg tggatcacca
      181 cggacaggtc ctgctcccgc accaggatga agtccgtctg gtaagtggac agcatcagca
      241 cagacacgtg gtgctcggcc agtggcgcga tgaccgaacg ggcgatcttg gtgaccccag
      301 cagcctgcac tgccgcaccg ctgtgagacg acacgttcag caccagccat gtggcctcag
      361 ctacttgcag gaactcagat gggggcagct ctttaaagcc ctcctcgtcc accataagcg
      421 tgtaatcctc aggggtctcc gtcaggctga agaacttgca ccggctgcgg cggggcagga
      481 agagcagctt gatgagcggg tgggtgtaga gccagagacc gggacgggcg acgctcagca
      541 cccgcacccg gtgttctagg atgtgcagct ccatcgtcga cgcaccacca ccaccaccct
      601 tgtcatcgtc atccttgtag tccatttcca tggtgaattc aatcgataga accgaggtgc
      661 agttggacct gggagtggac acctgtggag agaaaggcaa agtggatgtt attctatagt
      721 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
      781 acgaagccaa gggggtgggc ctatagactc tataggcggt acttacgtca ctcttggcac
      841 ggggaatccg cgttccaatg caccgttccc ggccgcggag gctggatcgg tcccggtgtc
      901 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
      961 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaa
//
