LOCUS       sequence-163553-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 ACT2 domain (aa 169-end).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-HA CASTOR1 ACT2
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        196..214
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     196..213
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     227..246
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      331..340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             343..369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      919..928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    932..1013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(969..988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
       61 acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt
      121 ctataggccc acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg
      181 tatcatacac atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc
      241 acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat tcccgaaccg
      301 acagtcggtc tcttcaccaa ggccattcgc gccaccatgg gctaccccta tgatgtgcct
      361 gactacgcag atctcaatgg tggtggtggt gggtcgacgg ggcccagccc cacggtgcat
      421 cccatccaga gcccacagaa ccgcttctgt gtcctcacac tggaccctga gacgcttcca
      481 gccatcgcca ccaccctcat agatgtcctc ttctactcgc acagcacccc caaggaggca
      541 gcctctagca gtcctgaacc cagctccatc acgttctttg ccttctctct catcgagggt
      601 tatatctcca ttgtcatgga tgctgaaaca cagaaaaagt tccccagtga cctcctgctg
      661 accagctcct cgggggagct gtggaggatg gtgcgcatcg gtggacagcc cctgggcttt
      721 gatgaatgtg gcatcgtggc acagattgca ggtcccctgg ctgccgctga catctctgcc
      781 tactacatca gcaccttcaa cttcgaccac gccctggtgc ccgaggacgg tatcggcagc
      841 gtcatcgagg tcctccagcg gcggcaggaa ggcctggctt cctgagcggc cgctaagtaa
      901 gtaaggatcc ccagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta
      961 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
//
