LOCUS       sequence-163554-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 ACT2 domain (aa 169-end).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-HA CASTOR1 ACT2
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(579..605)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      608..617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(702..721)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(734..752)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(735..752)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(962..986)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agctgcataa caagttgggc catggcggcc aagctgggga tccttactta cttagcggcc
       61 gctcaggaag ccaggccttc ctgccgccgc tggaggacct cgatgacgct gccgataccg
      121 tcctcgggca ccagggcgtg gtcgaagttg aaggtgctga tgtagtaggc agagatgtca
      181 gcggcagcca ggggacctgc aatctgtgcc acgatgccac attcatcaaa gcccaggggc
      241 tgtccaccga tgcgcaccat cctccacagc tcccccgagg agctggtcag caggaggtca
      301 ctggggaact ttttctgtgt ttcagcatcc atgacaatgg agatataacc ctcgatgaga
      361 gagaaggcaa agaacgtgat ggagctgggt tcaggactgc tagaggctgc ctccttgggg
      421 gtgctgtgcg agtagaagag gacatctatg agggtggtgg cgatggctgg aagcgtctca
      481 gggtccagtg tgaggacaca gaagcggttc tgtgggctct ggatgggatg caccgtgggg
      541 ctgggccccg tcgacccacc accaccacca ttgagatctg cgtagtcagg cacatcatag
      601 gggtagccca tggtggcgcg aatggccttg gtgaagagac cgactgtcgg ttcgggaatt
      661 caatcgatag aaccgaggtg cagttggacc tgggagtgga cacctgtgga gagaaaggca
      721 aagtggatgt tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
      781 attaattgta gccgcgttct aacgaagcca agggggtggg cctatagact ctataggcgg
      841 tacttacgtc actcttggca cggggaatcc gcgttccaat gcaccgttcc cggccgcgga
      901 ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
      961 cgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgc
//
