LOCUS       sequence-163560-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CYYR1_pCSdest
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..36
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(538..562)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    606..740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(630..649)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     684..703
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 attcaaggat cacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcaccatgg
       61 acgctccgag gctacccgtg cgtccagggg tcttgcttcc gaagttggtc ctgctctttg
      121 tctacgcaga tgattgcctt gctcagtgtg gcaaagattg caaatcttac tgctgtgatg
      181 gaaccacgcc ctactgttgc tcctactacg cttatattgg gaatatcctc tcgggcactg
      241 caattgcggg cattgttttt ggaatagtat ttatcatggg ggtcattgct gggattgcca
      301 tatgcatctg catgtgcatg aagaaccaca gggcgacccg cgtgggcatc ctcaggacga
      361 ctcacatcaa caccgtctcc tcctatcctg gaccaccacc ctacggtcac gaccacgaga
      421 tggaatactg tgcagacttg cctcctccat actcccccac cccacagggt ccagcacagc
      481 gttctccacc ccctccttat cctggaaacg caaggaaata aaagggtggg cgcgccgacc
      541 cagctttctt gtacaaagtg gtgatcctct cgagcctcta gaactatagt gagtcgtatt
      601 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      661 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      721 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
      781 gggaggtgtg ggaggttttt ta
//
