LOCUS       sequence-163561-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CYYR1_pCSdest
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(564..588)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(642..660)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(643..660)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gtcggcgcgc ccaccctttt atttccttgc gtttccagga taaggagggg gtggagaacg
      121 ctgtgctgga ccctgtgggg tgggggagta tggaggaggc aagtctgcac agtattccat
      181 ctcgtggtcg tgaccgtagg gtggtggtcc aggataggag gagacggtgt tgatgtgagt
      241 cgtcctgagg atgcccacgc gggtcgccct gtggttcttc atgcacatgc agatgcatat
      301 ggcaatccca gcaatgaccc ccatgataaa tactattcca aaaacaatgc ccgcaattgc
      361 agtgcccgag aggatattcc caatataagc gtagtaggag caacagtagg gcgtggttcc
      421 atcacagcag taagatttgc aatctttgcc acactgagca aggcaatcat ctgcgtagac
      481 aaagagcagg accaacttcg gaagcaagac ccctggacgc acgggtagcc tcggagcgtc
      541 catggtgaag ggggcggccg cggagcctgc ttttttgtac aaacttgtga tccttgaatt
      601 cgaatcgatg ggatcctgca aaaagaacaa gtagcttgta ttctatagtg tcacctaaat
      661 caagcttgct ccgacgtccc caggcagaat ggcggttccc taaacgagca ttgcttatat
      721 agacctccca ttaggcacgc ctaccgccca tttacgtcaa tggaacgccc atttgcgtca
      781 ttgcccctcc ccattgacgt caatggggat gtacttggca gccatcgcgg
//
