LOCUS       sequence-163563-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 ACT1 domain (aa 1-171).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-Flag CASTOR2 ACT1
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        196..214
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     196..213
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     227..246
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             305..328
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      902..911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
       61 acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt
      121 ctataggccc acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg
      181 tatcatacac atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc
      241 acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat tcaccatgga
      301 aatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga cgatggaact
      361 ccacatcctg gagcaccggc tgcaagttgc cagcgtcgcc aaggagagta tcccgctgtt
      421 cacctacggc ctgatcaaac ttgccttcct gtcctccaag accaggtgca agttcttcag
      481 tctgactgag acgccagagg attacactat cattgtcgat gaggaaggat tcctagagct
      541 gccctcctcg gagcacctga gtgtggcaga tgccacctgg ctggccctga acgtggtgtc
      601 cggcggtggc agcttctcca gctcccagcc catcggcgtg accaagatcg ccaagtcagt
      661 catcgcccca ctggctgacc agaacatatc cgtgttcatg ctgtccacgt atcagacaga
      721 cttcatcctg gtgcgcgagc gggacctgcc ctttgtcacc cacacattgt catcagagtt
      781 caccatcctg cgggtcgtca atggcgagac cgtggcagcc gagaacctcg gcatcaccaa
      841 tggcttcgtg aagcccaagc tggtctgagc ggccgctaag taagtaagga tccccagctt
      901 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      961 tcacaa
//
