LOCUS       sequence-163567-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 ACT1 domain (aa 1-171).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-Flag CASTOR2 ACT1
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(605..628)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(687..706)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(719..737)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(720..737)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(947..971)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agctgcaata aacaagttgg gccatggcgg ccaagctggg gatccttact tacttagcgg
       61 ccgctcagac cagcttgggc ttcacgaagc cattggtgat gccgaggttc tcggctgcca
      121 cggtctcgcc attgacgacc cgcaggatgg tgaactctga tgacaatgtg tgggtgacaa
      181 agggcaggtc ccgctcgcgc accaggatga agtctgtctg atacgtggac agcatgaaca
      241 cggatatgtt ctggtcagcc agtggggcga tgactgactt ggcgatcttg gtcacgccga
      301 tgggctggga gctggagaag ctgccaccgc cggacaccac gttcagggcc agccaggtgg
      361 catctgccac actcaggtgc tccgaggagg gcagctctag gaatccttcc tcatcgacaa
      421 tgatagtgta atcctctggc gtctcagtca gactgaagaa cttgcacctg gtcttggagg
      481 acaggaaggc aagtttgatc aggccgtagg tgaacagcgg gatactctcc ttggcgacgc
      541 tggcaacttg cagccggtgc tccaggatgt ggagttccat cgtcgacgca ccaccaccac
      601 cacccttgtc atcgtcatcc ttgtagtcca tttccatggt gaattcaatc gatagaaccg
      661 aggtgcagtt ggacctggga gtggacacct gtggagagaa aggcaaagtg gatgttattc
      721 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
      781 gttctaacga agccaagggg gtgggcctat agactctata ggcggtactt acgtcactct
      841 tggcacgggg aatccgcgtt ccaatgcacc gttcccggcc gcggaggctg gatcggtccc
      901 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
      961 ctaaacgagc tctgc
//
