LOCUS       sequence-163572-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78C protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78C
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             334..351
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(416..434)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      430..477
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      514..969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(601..620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     811..832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatatg gctagctcct ccgactctcc gcgtaacctg
       61 gaggttacca acgcaactcc gaactctctg actatttctt gggacgatcc ttatcacgct
      121 atctattacc gtatcaccta cggcgaaact ggtggtaact ccccgagcca ggaattcact
      181 gttccgagat cttattctaa tgcgaccatc agcggtctga aaccgggcca ggattatacc
      241 attaccgtgt acgctgtaac cggttgtaac gacgattcaa acccaatcag catcaattat
      301 cgcaccgaaa tcgacaaacc gtctcaggga tcccaccatc accatcatca ctgattaact
      361 aaacgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      421 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      481 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      541 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      601 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      661 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      721 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      781 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      841 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      901 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      961 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1021 ctaaatacat tcaaatatgt atccgctca
//
