LOCUS       sequence-163577-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 ACT2 domain (aa 171-end).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-HA CASTOR2 ACT2
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        196..214
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     196..213
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     227..246
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      331..340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             343..369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      913..922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    926..1007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(963..982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
       61 acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt
      121 ctataggccc acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg
      181 tatcatacac atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc
      241 acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat tcccgaaccg
      301 acagtcggtc tcttcaccaa ggccattcgc gccaccatgg gctaccccta tgatgtgcct
      361 gactacgcag atctcaatgg tggtggtggt gggtcgacgg tccagaggcc agtcatccac
      421 ccactgtcca gcccgagcaa caggttctgt gtcaccagcc tggaccctga cacgctgcct
      481 gctgttgcca cactcctcat ggatgtcatg ttctactcca atggagtgaa ggaccccatg
      541 gccactgggg atgactgcgg ccacatccgc ttcttctcct tctccctcat cgagggctac
      601 atctccctgg tgatggacgt gcagacgcag cagaggtttc ctagtaactt gctgttcaca
      661 agcgcatccg gagagctctg gaagatggtc cggattggag gacagcccct ggggtttgat
      721 gagtgtggca tcgtggccca gatctcagag cccttggctg ctgcagacat cccagcctac
      781 tacatcagta ctttcaagtt tgatcatgca cttgtccccg aagagaacat caatggtgtc
      841 atcagtgccc tgaaggtcag ccaagcagag aagcactgag cggccgctaa gtaagtaagg
      901 atccccagct tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata
      961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1021 tt
//
