LOCUS       sequence-163583-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR2 ACT2 domain (aa 171-end).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-HA CASTOR2 ACT2
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(574..600)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      603..612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(697..716)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(729..747)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(730..747)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(957..981)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(978..1016)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gctgcaataa acaagttggg ccatggcggc caagctgggg atccttactt acttagcggc
       61 cgctcagtgc ttctctgctt ggctgacctt cagggcactg atgacaccat tgatgttctc
      121 ttcggggaca agtgcatgat caaacttgaa agtactgatg tagtaggctg ggatgtctgc
      181 agcagccaag ggctctgaga tctgggccac gatgccacac tcatcaaacc ccaggggctg
      241 tcctccaatc cggaccatct tccagagctc tccggatgcg cttgtgaaca gcaagttact
      301 aggaaacctc tgctgcgtct gcacgtccat caccagggag atgtagccct cgatgaggga
      361 gaaggagaag aagcggatgt ggccgcagtc atccccagtg gccatggggt ccttcactcc
      421 attggagtag aacatgacat ccatgaggag tgtggcaaca gcaggcagcg tgtcagggtc
      481 caggctggtg acacagaacc tgttgctcgg gctggacagt gggtggatga ctggcctctg
      541 gaccgtcgac ccaccaccac caccattgag atctgcgtag tcaggcacat cataggggta
      601 gcccatggtg gcgcgaatgg ccttggtgaa gagaccgact gtcggttcgg gaattcaatc
      661 gatagaaccg aggtgcagtt ggacctggga gtggacacct gtggagagaa aggcaaagtg
      721 gatgttattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
      781 ttgtagccgc gttctaacga agccaagggg gtgggcctat agactctata ggcggtactt
      841 acgtcactct tggcacgggg aatccgcgtt ccaatgcacc gttcccggcc gcggaggctg
      901 gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc
      961 tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgcc
//
