LOCUS       sequence-163586-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78D protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78D
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             343..360
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(425..443)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      439..486
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      523..978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(610..629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     820..841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg ctagctcctc cgactctccg
       61 cgtaacctgg aggttaccaa cgcaactccg aactctctga ctatttcttg ggacgatcct
      121 tatcacgcta tctattaccg tatcacctac ggcgaaactg gtggtaactc cccgagccag
      181 gaattcactg ttccgagatc ttattctaat gcgaccatca gcggtctgaa accgggccag
      241 gattatacca ttaccgtgta cgctgtaacc ggtgataacg acgattcaaa cccaatcagc
      301 atcaattatc gcaccgaaat cgacaaaccg tctcagggat cccaccatca ccatcatcac
      361 tgattaacta aacgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      421 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      481 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      541 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      601 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      661 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      721 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      781 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      841 gaacaacact caaccctatc tccgtctatt cttttgattt ataagggatt ttgccgattt
      901 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      961 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1021 tttatttttc taaatacatt caaatatgta tccgctca
//
