LOCUS       sequence-163590-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 I280A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-Flag CASTOR1 I280A
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        196..214
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     196..213
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     227..246
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      299..308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
       61 acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt
      121 ctataggccc acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg
      181 tatcatacac atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc
      241 acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat tcgtcgacgc
      301 caccatggag ctgcacatcc tagaacaccg ggtgcgggtg ctgagcgtcg cccgtcccgg
      361 tctctggctc tacacccacc cgctcatcaa gctgctcttc ctgccccgcc gcagccggtg
      421 caagttcttc agcctgacgg agacccctga ggattacacg cttatggtgg acgaggaggg
      481 ctttaaagag ctgcccccat ctgagttcct gcaagtagct gaggccacat ggctggtgct
      541 gaacgtgtcg tctcacagcg gtgcggcagt gcaggctgct ggggtcacca agatcgcccg
      601 ttcggtcatc gcgccactgg ccgagcacca cgtgtctgtg ctgatgctgt ccacttacca
      661 gacggacttc atcctggtgc gggagcagga cctgtccgtg gtgatccaca cgctggccca
      721 ggagttcgac atttaccgcg aggtgggcgg agagcctgtg cctgtgacga gggatgattc
      781 cagcaatggc tttccccgca ctcagcatgg gcccagcccc acggtgcatc ccatccagag
      841 cccacagaac cgcttctgtg tcctcacact ggaccctgag acgcttccag ccatcgccac
      901 caccctcata gatgtcctct tctactcgca cagcaccccc aaggaggcag cctctagcag
      961 tcctgaaccc agctccatca cgttctttgc cttctctctc atcgagggtt ata
//
