LOCUS       sequence-163591-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 I280A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5-Flag CASTOR1 I280A
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(52..75)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctgcaata aacaagttgg gccatggcgg ccaagctggg gatccttatc acttgtcatc
       61 gtcatccttg tagtcacctc cacctcctgc ggccgcggaa gccaggcctt cctgccgccg
      121 ctggaggacc tcgatgacgc tgccgatacc gtcctcgggc accagggcgt ggtcgaagtt
      181 gaaggtgctg atgtagtagg cagagatgtc agcggcagcc aggggacctg caatctgtgc
      241 cacggcgcca cattcatcaa agcccagggg ctgtccaccg atgcgcacca tcctccacag
      301 ctcccccgag gagctggtca gcaggaggtc actggggaac tttttctgtg tttcagcatc
      361 catgacaatg gagatataac cctcgatgag agagaaggca aagaacgtga tggagctggg
      421 ttcaggactg ctagaggctg cctccttggg ggtgctgtgc gagtagaaga ggacatctat
      481 gagggtggtg gcgatggctg gaagcgtctc agggtccagt gtgaggacac agaagcggtt
      541 ctgtgggctc tggatgggat gcaccgtggg gctgggccca tgctgagtgc ggggaaagcc
      601 attgctggaa tcatccctcg tcacaggcac aggctctccg cccacctcgc ggtaaatgtc
      661 gaactcctgg gccagcgtgt ggatcaccac ggacaggtcc tgctcccgca ccaggatgaa
      721 gtccgtctgg taagtggaca gcatcagcac agacacgtgg tgctcggcca gtggcgcgat
      781 gaccgaacgg gcgatcttgg tgaccccagc agcctgcact gccgcaccgc tgtgagacga
      841 cacgttcagc accagccatg tggcctcagc tacttgcagg aactcagatg ggggcagctc
      901 tttaaagccc tcctcgtcca ccataagcgt gtaatcctca ggggtctccg tcaggctgaa
      961 gaacttgcac cggctgcggc ggggcaggaa gagcagcttg atgagcgggt gggtgt
//
