LOCUS       sequence-163596-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the nDHFR-link1-His6-Ub, UBCH5B Hs and UBA1Ta in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pND-Ub16-UBCH5B Hs
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             71..82
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             527..544
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             566..586
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tagagattga catccctatc agtgatagag atactgagca catcagcagg acgcactgac
       61 cgaattcatt aaagaggaga aaggtaccat ggttcgacca ttgaactgca tcgtcgccgt
      121 gtcccaaaat atggggattg gcaagaacgg agacctaccc tggcctccgc tcaggaacga
      181 gttcaagtac ttccaaagaa tgaccacaac ctcttcagtg gaaggtaaac agaatctggt
      241 gattatgggt aggaaaacct ggttctccat tcctgagaag aatcgacctt taaaggacag
      301 aattaatata gttctcagta gagaactcaa agaaccacca cgaggagctc attttcttgc
      361 caaaagtttg gatgatgcct taagacttat tgaacaaccg gaattggcat taattaaggc
      421 ggctcagcgg gctcgggaag cggagcggga tctggcagcg gcggtagcgc aggcagcagc
      481 gggtcaagcg gtgcctcgag cggcgcggcc gcaaatgtcg tactaccatc accatcacca
      541 tcacgattac gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tgggatccat
      601 gcagatcttc gtgaagactc tgactggtaa gaccatcacc ctcgaggtgg agcccagtga
      661 caccatcgag aatgtcaagg caaagatcca agataaggaa ggcattcctc ctgatcagca
      721 gaggttgatc tttgccggaa aacagctgga agatggtcgt accctgtctg actacaacat
      781 ccagaaagag tccaccttgc acctggtact ccgtctcaga g
//
