LOCUS       sequence-163597-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of CASTOR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pLJC6-Flag CASTOR1
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    131..248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(296..319)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctgcgtttcc ggaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg
       61 gaaaaggcgc aaccccaacc ccctcgagcc gcggccaaag tggatctctg ctgtccctgt
      121 aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt
      181 ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc
      241 ttttaaaatt gtggatgaat actgccattt gtctcgaggt cgagaattct tatcacttgt
      301 catcgtcatc cttgtagtca cctccacctc ctgcggccgc ggaagccagg ccttcctgcc
      361 gccgctggag gacctcgatg acgctgccga taccgtcctc gggcaccagg gcgtggtcga
      421 agttgaaggt gctgatgtag taggcagaga tgtcagcggc agccagggga cctgcaatct
      481 gtgccacgat gccacattca tcaaagccca ggggctgtcc accgatgcgc accatcctcc
      541 acagctcccc cgaggagctg gtcagcagga ggtcactggg gaactttttc tgtgtttcag
      601 catccatgac aatggagata taaccctcga tgagagagaa ggcaaagaac gtgatggagc
      661 tgggttcagg actgctagag gctgcctcct tgggggtgct gtgcgagtag aagaggacat
      721 ctatgagggt ggtggcgatg gctggaagcg tctcagggtc cagtgtgagg acacagaagc
      781 ggttctgtgg gctctggatg ggatgcaccg tggggctggg cccatgctga gtgcggggaa
      841 agccattgct ggaatcatcc ctcgtcacag gcacaggctc tccgcccacc tcgcggtaaa
      901 tgtcgaactc ctgggccagc gtgtggatca ccacggacag gtcctgctcc cgcaccagga
      961 tgaagtccgt ctggtaagtg gacagcatca gcacagacac gtggtgctcg gccagtggcg
     1021 cgatgac
//
