LOCUS       sequence-163609-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pSc-His6-Sem1Sc
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..42)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(270..287)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(384..825)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(390..413)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    708..825
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(865..885)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(865..883)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(904..920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(904..920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cactgatttt cccgtttgta tctatccaat tcggccttca attcattggt gaaatcgtcg
       61 tcaacttcaa catcatccca gttttcttcc catatgtttg tttgcgttac agcattgctt
      121 tttattgttt caccattggc ccatgtgtca atggggaaat cttcaaattc atcgtcttcc
      181 tctagagact tcttatttat ttcctcattt tttttttttg ttaaatctat tttactttgg
      241 gcttgtgcag cagcaacgtc cgtactcatg tggtgatgat ggtgatggct gctgcccatg
      301 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagtgcgg
      361 ccgcttcaga atccggggtt tttctccttg acgttaaagt atagaggtat attaacaatt
      421 ttttgttgat acttttatta catttgaata agaagtaata caaaccgaaa atgttgaaag
      481 tattagttaa agtggttatg cagtttttgc atttatatat ctgttaatag atcaaaaatc
      541 atcgcttcgc tgattaatta ccccagaaat aaggctaaaa aactaatcgc attatcatcc
      601 tatggttgtt aatttgattc gttcatttga aggtttgtgg ggccaggtta ctgccaattt
      661 ttcctcttca taaccataaa agctagtatt gtagaatctt tattgttcgg agcagtgcgg
      721 cgcgaggcac atctgcgttt caggaacgcg accggtgaag acgaggacgc acggaggaga
      781 gtcttccttc ggagggctgt cacccgctcg gcggcttcta atccgtacta ggcgcgccgt
      841 ttaaacgagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      901 atggtcatag ctgtttcctg tgtgaaa
//
