LOCUS       sequence-163611-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCDF-His6-MBP-Sem1 Sc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCOG75-Sem1 Sc
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             277..288
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     397..416
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        397..415
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    416..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             549..593
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(631..649)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      645..692
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctcgggatc gatacgaccg aaaacctgta ttttcagggc gggcgcgcca tgagtacgga
       61 cgttgctgct gcacaagccc aaagtaaaat agatttaaca aaaaaaaaaa atgaggaaat
      121 aaataagaag tctctagagg aagacgatga atttgaagat ttccccattg acacatgggc
      181 caatggtgaa acaataaaaa gcaatgctgt aacgcaaaca aacatatggg aagaaaactg
      241 ggatgatgtt gaagttgacg acgatttcac caatgaattg aaggccgaat tggatagata
      301 caaacgggaa aatcagtgag tcgacaagct tgcggccgca taatgcttaa gtcgaacaga
      361 aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact ataggggaat
      421 tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac
      481 atatggcaga tctcaattgg atatcggccg gccacgcgat cgctgacgtc ggtaccctcg
      541 agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta
      601 gcgcagctta attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg
      661 gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca
      721 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
      781 tttaacgacc ctgccctgaa ccgacgaccg ggtcatcgtg gccggatctt gcggcccctc
      841 ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
      901 agtggacaaa ttcttccaac tgatctgcgc gcgaggcc
//
