LOCUS       sequence-163621-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of CASTOR2 I280A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pLJC6-Flag CASTOR1 I280A
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(297..320)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctgcgtttcc ggaaccacgc ccagagcagc cgcgtccctg cgcaaaccca gggctgcctt
       61 ggaaaaggcg caaccccaac cccctcgagc cgcggccaaa gtggatctct gctgtccctg
      121 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
      181 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
      241 cttttaaaat tgtggatgaa tactgccatt tgtctcgagg tcgagaattc ttatcacttg
      301 tcatcgtcat ccttgtagtc acctccacct cctgcggccg cggaagccag gccttcctgc
      361 cgccgctgga ggacctcgat gacgctgccg ataccgtcct cgggcaccag ggcgtggtcg
      421 aagttgaagg tgctgatgta gtaggcagag atgtcagcgg cagccagggg acctgcaatc
      481 tgtgccacgg cgccacattc atcaaagccc aggggctgtc caccgatgcg caccatcctc
      541 cacagctccc ccgaggagct ggtcagcagg aggtcactgg ggaacttttt ctgtgtttca
      601 gcatccatga caatggagat ataaccctcg atgagagaga aggcaaagaa cgtgatggag
      661 ctgggttcag gactgctaga ggctgcctcc ttgggggtgc tgtgcgagta gaagaggaca
      721 tctatgaggg tggtggcgat ggctggaagc gtctcagggt ccagtgtgag gacacagaag
      781 cggttctgtg ggctctggat gggatgcacc gtggggctgg gcccatgctg agtgcgggga
      841 aagccattgc tggaatcatc cctcgtcaca ggcacaggct ctccgcccac ctcgcggtaa
      901 atgtcgaact cctgggccag cgtgtggatc accacggaca ggtcctgctc ccgcaccagg
      961 atgaagtccg tctggtaagt ggacagcatc agc
//
