LOCUS       sequence-163624-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78I protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78I
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..55)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             369..391
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    406..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(430..449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(431..449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(533..552)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        758..835
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(855..874)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tctcgtttag ttaatcagtg atgatggtga tggtgggatc
       61 cctgagacgg tttgtcgatt tcggtgcgat aattgatgct gattgggttt gaatcgtcgt
      121 taataccggt tacagcgtac acggtaatgg tataatcctg gcccggtttc agaccgctga
      181 tggtcgcatt agaataagat ctcggaacag tgaattcctg gctcggggag ttaccaccag
      241 tttcgccgta ggtgatacgg taatagatag cgtgataagg atcgtcccaa gaaatagtca
      301 gagagttcgg agttgcgttg gtaacctcca ggttacgcgg agagtcggag gagctagcca
      361 tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      421 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      481 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      541 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      601 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      661 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      721 atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac
      781 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
      841 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
      901 cgtggtgaac caggccagcc acgtttct
//
