LOCUS       sequence-163645-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control shRNA pLVTHM-shN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pLVTHM-shN
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(112..145)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             165..345
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(370..388)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(371..388)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(678..697)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(911..930)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgtctttgga tttgggaatc ttataagttc tgtatgagac cacgcgtccc cgtgcgttgc
       61 tagtaccaac ttcaagagat tttttacgcg tatcgatacc gtcgcatggg aataacttcg
      121 tatagcatac attatacgaa gttatgctgc tttttgcttg tactgggtct ctctggttag
      181 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      241 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      301 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
      361 tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
      421 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
      481 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
      541 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
      601 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      661 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      721 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      781 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      841 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
      901 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      961 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1021 tgaccgtc
//
