LOCUS       sequence-163661-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mst2 SARAH domain for interaction and spectroscopy 
            studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Rawat SJ, Araiza-Olivera D, Arias-Romero LE, Villamar-Cruz O, 
            Prudnikova TY, Roder H, Chernoff J
  TITLE     H-ras Inhibits the Hippo Pathway by Promoting Mst1/Mst2 
            Heterodimerization.
  JOURNAL   Curr Biol. 2016 Jun 20;26(12):1556-63. doi: 
            10.1016/j.cub.2016.04.027. Epub 2016 May 26.
  PUBMED    27238285
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2016.04.027"; journalName: "Curr Biol"; date: 
            "2016-06-20- 20"; volume: "26"; issue: "12"; pages: "1556-63"
COMMENT     Sequence Label: pGEX4T-Mst2 SARAH
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(39..143)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     211..229
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     374..396
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(636..659)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(686..708)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
       61 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
      181 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
      241 caagaattat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
      301 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      361 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      421 gcgaggcaga tcgtcagtca gtcacgatgc ggccgctcga gtcgacccgg gaattcgcac
      481 tttgcatcca tcgcatccag aatgggctgt cttttcgcag tgtatctctg acgaagttct
      541 tctatctccc gttccatcat ggggtccagt gcttttaacc gcatctgtag ttcttctaaa
      601 ctccaatttt tcaaaaagtc aaagtcggat cccaggggcc cctggaacag aacttccaga
      661 tccgattttg gaggatggtc gccaccacca aacgtggctt gccagccctg caaaggccat
      721 gctatatact tgctggatt
//
