LOCUS       sequence-163668-       10604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 
            selection. tdTomato was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and synthetic introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 10604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1955
FEATURES             Location/Qualifiers
     source          1..10604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3568..3588
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3589..4188
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    4214..4238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6295..6366
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(6368..6392)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      6397..6720
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(6724..6744)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6752..6769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6752..6768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8470..8489)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8589..8611
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8649..8667)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8735..8839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8840..8908,8909..9700)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9058..9077)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9871..10459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10360..10379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca
     3541 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttggc acctttggtc
     3601 ttttattgtc aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat
     3661 aaaataaaga cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga
     3721 gaaaaagtat gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg
     3781 ctaggagtta gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa
     3841 attaacgtga gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg
     3901 tttttatctt tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt
     3961 caaacattca gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg
     4021 ctttcaacaa atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt
     4081 ttaaaaatat tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc
     4141 actcttctta cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctcactc
     4201 cgtagcagcc atggcaagtt tgtacaaaaa agcaggctcg ccaaaggtaa gtttatctaa
     4261 aaagtttttc attcaaaatg tgtaaaaatt catttaaaat aacccaaaaa tcattaatcc
     4321 tcgatatttt cagaagaagc gtaaggtcgt ttccaaggga gaggaggtat tttcctgcat
     4381 ttttcaactg ggaaaatgaa agaaaatcga taatttcagg acaacatggc cgtcatcaag
     4441 gagttcatgc gtttcaaggt ccgtatggag ggatccatga acggacacga gttcgagatc
     4501 gaaggagagg gtgagggccg tccatacgag ggaacccaaa ccgccaagct caaggtaagt
     4561 tttctagaga cctttactaa ctaactaggt ctcatagatt ttcaggtcac caagggagga
     4621 ccactcccat tcgcctggga catcctctcc ccacaattca tgtacggatc caaggcctac
     4681 gtcaagcacc cagccgacat cccagactac aagaagctct ccttcccagg taagttatgt
     4741 agagaccttt actaactaac taggtctcaa acattttcag agggattcaa gtgggagcgt
     4801 gtcatgaact tcgaggacgg aggactcgtc accgtcaccc aagactcctc cctccaagac
     4861 ggaaccctca tctacaaggt caagatgcgt ggaaccaact tcccaccagg taagttcttt
     4921 agagaccttt actaactaac taggtctcag taattttcag acggaccagt catgcaaaag
     4981 aagaccatgg gatgggaggc ctccaccgag cgcctctacc cacgtgacgg agtcctcaag
     5041 ggagagatcc accaagccct caagctcaag gtaagttcat aagagacctt tactaactaa
     5101 ctaggtctca gaatttttca ggacggagga cactacctcg tcgagttcaa gaccatctac
     5161 atggccaaga agccagtcca actcccagga tactactacg tcgacaccaa gctcgacatc
     5221 acctcccaca acgaggacta caccatcgtc gagcaatacg agcgttccga gggacgtcac
     5281 cacctcttcc tcggacacgg aaccggatcc accggatccg gatcctccgg aaccgcctcc
     5341 tccgaggaca acaacatggc cgtcatcaag gtaagttttc tagagacctt tactaactaa
     5401 ctaggtctca tagattttca ggagttcatg cgtttcaagg tccgtatgga gggatccatg
     5461 aacggacacg agttcgagat cgagggagaa ggcgaaggac gtccatacga gggaacccaa
     5521 accgccaagc tcaaggtcac caaggtaagt tatgtagaga cctttactaa ctaactaggt
     5581 ctcaaacatt ttcagggagg accactccca ttcgcctggg acatcctctc cccacaattc
     5641 atgtacggat ccaaggccta cgtcaagcac ccagccgaca tcccagacta caaggtaagt
     5701 tctttagaga cctttactaa ctaactaggt ctcagtaatt ttcagaagct ctccttccca
     5761 gagggattca agtgggagcg tgtcatgaac ttcgaggacg gaggactcgt caccgtcacc
     5821 caagactcct ccctccaaga cggaaccctc atatacaaag tcaaggtaag ttcataagag
     5881 acctttacta actaactagg tctcagaatt tttcagatgc gtggaaccaa cttcccacca
     5941 gacggaccag tcatgcaaaa gaagaccatg ggatgggagg cctccaccga gcgcctctac
     6001 ccacgtgacg gagtcctcaa gggagagatc caccaagccc tcaagctcaa ggtaagtttt
     6061 ctagagacct ttactaacta actaggtctc atagattttc aggacggagg acactacctc
     6121 gtcgagttca agaccatcta catggccaag aagccagtcc aactcccagg atactactac
     6181 gtcgacacca agctcgacat cacctcccac aacgaggact acaccatcgt cgagcaatac
     6241 gagcgttccg agggacgtca ccacctcttc ctcggaatgg acgagctcta caagtcccgt
     6301 cgtcgtaagg ccaacccaac caagctctcc gagaacgcca agaagctcgc caaggaggtc
     6361 gagaacaacc cagctttctt gtacaaagtg ggataaatgc aaaatccttt caagcattcc
     6421 cttcttctct atcactcttc tttctttttg tcaaaaaatt ctctcgctaa tttatttgct
     6481 tttttaatgt tattatttta tgacttttta tagtcactga aaagtttgca tctgagtgaa
     6541 gtgaatgcta tcaaaatgtg attctgtctg atgtactttc acaatctctc ttcaattcca
     6601 ttttgaagtg ctttaaaccc gaaaggttga gaaaaatgcg agcgctcaaa tatttgtatt
     6661 gtgttcgttg agtgacccaa caaaaagagg aaactttatt gtgccgccaa gaaaaaagtc
     6721 tcacaacttt attatacata gttgataatt cactggccgt cgttttacac tcgagacgta
     6781 cggtgcgcgc gatgcattcg aagatctgcc cactagtgag tcgtattata agtgcaagta
     6841 agatcagtgt ttgtttcgat atcagtctgt ttcgtaacgg tcttctgtat aactacaaaa
     6901 aagaataaaa aaccgtatca caactgagaa aaaagacaaa acttattgaa cagtgttcct
     6961 ttcaaaaaat tattttgtaa aaaatcgagt ttttctccaa aataacgcac gcaatcaaaa
     7021 acaagaaaac taacgtgttc tcctgggcgt ggaactcctt atcaagatta ccattattgt
     7081 ctgtcagaat tgatatttca attatttcat atctagacaa acccgggtgt cttgcattta
     7141 ttagtactgt gagtttacat acaaacgagg cttactcaga taacatagag tcacctctaa
     7201 ttagcaaaaa cccatttcta attagctatt tcgaccagtt ctaaagtcac cgtaattaga
     7261 aatggttttt gtagcttttc taattagaaa tgaccccgag ataagtgaaa tatggcattt
     7321 tctgggtttt ctagtaagaa atgatcccgc acgtagtgat tccgcgttac aagccaaatt
     7381 caaattaaaa aagtatatta cacaattcaa gttacattat tttcaggaaa caatggcgaa
     7441 gagcaaagca gagattcaaa aggaggaaag agacaacaga tatgaacgaa gacttcttga
     7501 aaagagaaag agacatgagg tgagtaacca tgataataaa taatcaagta agggctatag
     7561 atttataaat tctggagtta acagtttcga aaaaaagtag ttttcatatg tgcgaaggaa
     7621 tatcaaaaat aggggaatag cctaaacgag tgagaaaatt aaatgtttgt tattttttca
     7681 aaaatgtctg cagggccgtt atcctttcaa atgcactcta tcaaacatag atcatatcca
     7741 tcatatctta tccagaattt atatcatatc caagattgag agcgtttcaa ggaaaatctc
     7801 aaacaaccat acaattttgt agatcgacaa caaggcctcc acttcttcat cgatggcctc
     7861 tgcagcacct gcaccaccaa gtttgtattt ttcatattaa tataaagtga gccacgcata
     7921 gtcgttttta ggatttttgc cagatcgatg tcgcgcaaac agactgtttt tttttctttg
     7981 aaagccgtgc gtgacatggg ccacggattg taacgccgcg ctggatggat aaaaatttat
     8041 tcagtttttt agcctttaca aaatatggtt cgactacttt ctttattttt acatttttat
     8101 aggtgctcat tgagctgaga tattttgggt ataatcggaa aatcgctgat tttggtcatt
     8161 tttctgcact aaagattaca caaaaagttc agtatttaga ttcattgttg aaaattgggg
     8221 atgatctaca gatacatatc gccgtgttca aaaacacacg tgacgtcgtg actgggaaaa
     8281 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8341 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     8461 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     8521 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     8581 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     8641 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8701 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8761 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8821 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8881 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8941 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9001 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9061 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9121 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9181 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9241 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9301 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9361 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9421 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9481 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9541 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9601 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9661 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9721 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9781 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9841 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9901 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9961 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    10021 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10081 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10141 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10201 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10261 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10321 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10381 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10441 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggtcttttg
    10501 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10561 taccgccttt gagtgagctg ataccagcgg ataacaattt caca
//
