LOCUS       sequence-163669-       14806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 
            selection. GFP was optimized for C. elegans, contains 2x NLS 
            (SV40/egl-13), and smu-2 introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14806)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 14806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ1320
FEATURES             Location/Qualifiers
     source          1..14806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3568..3588
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3589..4188
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    4214..4238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4241..4261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10497..10568
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(10570..10594)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      10599..10922
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(10926..10946)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10954..10971)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10954..10970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12672..12691)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12791..12813
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12851..12869)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12937..13041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13042..13110,13111..13902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13260..13279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14073..14661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14562..14581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca
     3541 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttggc acctttggtc
     3601 ttttattgtc aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat
     3661 aaaataaaga cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga
     3721 gaaaaagtat gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg
     3781 ctaggagtta gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa
     3841 attaacgtga gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg
     3901 tttttatctt tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt
     3961 caaacattca gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg
     4021 ctttcaacaa atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt
     4081 ttaaaaatat tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc
     4141 actcttctta cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctcactc
     4201 cgtagcagcc atggcaagtt tgtacaaaaa agcaggctcg ccaaagaaga agcgtaaggt
     4261 gtccaaggta agttttctgt tgaacggctg aaaaatcgat attttgagcg aaaaaagccg
     4321 gaaaaatgga ttttatcgga taaaatttga ttttttgagc tgagaaatgc ctttatagac
     4381 gaatttcctt gtcaaattgt taaaaactgc aaaatttgcc aaaaaaaaag cattttttat
     4441 attttttttt acaaaaaatt ccacaaaaat ggcaaaattt gactaaaaat gccaattttg
     4501 tcgttttctc cgtgccacag cggccgaaaa tcgattttta agcgattttc gggtgaaaaa
     4561 gtgtaaaaac cgactgaaaa tccagctgaa aacgacaaaa acggtaagtt ttagctaatt
     4621 ttcacctttt tttcgatatt tttttactaa aaaaaaaccg aaaaattgag tttttttcaa
     4681 atttctgcaa aaattctgca attttagcaa tttccatact attttgacgc cgaaaaagct
     4741 taaaaatatg atttttagag gtttttaatg gaaaattcat ggatttttca aatttttcca
     4801 cgaaaatcat tgaaaaattt gaaaaaattg atttcctatt caaagttcta gctaaaaact
     4861 gcaattttag caatttccat actattttag tactgaaaaa gcctaaaaat gtgattttca
     4921 gaggatttca atggaaaatt catggatttt tccaattttt caatgaaaac cattgaaaaa
     4981 ccctggaaaa ttgagttttt cttcaaagtt taggaaattt atgagaattt ttgaataaaa
     5041 aatttttaaa tttgaaaaaa attgaaattt taattctaaa ttttaaagaa agttgatttt
     5101 ttaataattt ttttttttga ataaaaaaaa atcaactttc tttaaaattt agaattaaaa
     5161 tttcaatttt tttattcaaa aattctcata aatttcgtaa acttttaata aaaactcaat
     5221 ttttccgatt tttttcgatg attttcacta aaaaattgga aaaattcatc aatttcctat
     5281 tgaaaacctc taaaaattat attttttttg cttttccgac gtcaaaatag tatggaaaat
     5341 gctaaaattg cagaattttt ccagaaattt gaagaaaaaa ctcaattttt tccgtttttt
     5401 caatgatttt catgaaaaaa ttggaaaatt ttgtaaattt tccattgaaa acctctgaaa
     5461 atttggaaat tttcgaattt aaatgtttaa aaaaaattga atattttgcg agtttttgaa
     5521 ttttttttga gaatttttga atttttttca cccaaaattt tttcaattag attttcaggg
     5581 agaggagctc ttcaccggag tcgtcccaat cctcgtcgag ctcgacggag acgtcaacgg
     5641 acacaagttc tccgtctccg gagagggaga gggagacgcc acctacggaa agctcaccct
     5701 caagttcatc tgcaccaccg gaaagctccc agtcccatgg ccaaccctcg tcaccacctt
     5761 ctgctacgga gtccaatgct tctcccgtta cccagaccac atgaagcgtc acgacttctt
     5821 caagtccgcc atgccagagg gatacgtcca agagcgtacc atcttcttcg taagttatgt
     5881 agctcgaaaa tttggagctt ttacacgaga tttttgaaga aaaagcctga aaatcgagag
     5941 attttgagct gtggaattcg ttgaaattac ataattcgcg cccgtaattc tttaaaaaaa
     6001 cgctgaaatt tgcagatttt gccagaaagt tgaagaaaaa tctacttttc cggcattctc
     6061 tattattttc atggaaaatt agaaaaatcc atgaataaac agtgaaaatt gctgatttta
     6121 cctgaaattt tgaaaaaaaa aaaaatctaa aatcaaggtt ttaagcctaa aaactgcaga
     6181 tttcagccaa aattgtgaaa aaagctgatt ttttgctatt ttgagctttt ccatggggtg
     6241 gaaattgcca aaaattacgg aacattcaca caaatttgct gaataatcca attttccagt
     6301 gttttctaga gtttccgtag aaattttgaa aaaacccagt aaattttcaa tcggaaaacc
     6361 tctaaaaaca tcgtttcagg cctataaata gtaaaaatta ccgattttat tcgaaaattt
     6421 aaggaaaagt cgtgaaaaac gagattttaa gcctcaaaac tgcagattct atctaaaatt
     6481 aaaaaaaaaa aacataaata tccgaatttt tggaggaaaa gcctgaaaat cgatatttta
     6541 agcctcaaaa ctgaagattt cagccaaaaa aaaaaacaga tttttcgcta ttttatgtgc
     6601 caaaaaacca cattccgagc ttttccagag tggaaattgc caaaaattac agaattttcc
     6661 cagaaattag caaaaaaaaa acccattttt caggcgtttt caacgatttt catagcaaat
     6721 ttgaaaaatc catgaatttt ttaattatag aaaaccctct gaaaattgca gttttaagct
     6781 attccagcat tcaaatagta tggaaattgc taaaaattga agaattttcc caaaaatttg
     6841 atgaaaactt atttttctgg cgttttcgat gattttcatg gaatttcgag aaaattaatg
     6901 aattttcaat taaaaaacgt ctgcaaaatc acatttttta aagctttttc agcaacaaaa
     6961 tagtatggaa attgcttttt aaaaaagcgg aatttttgga aaaaaggtta aattttcagt
     7021 cagtttttgc actttttgac tgaaaattca ctgaaattag ggaaaaattc cggaaaaatg
     7081 attttttcga attgtagggc aaattttgca atttcaagca atttccataa tattttaatg
     7141 ctggaaaagc tcaaatttac gttttaatgg gattttagtg aaaaattcat ggattttccg
     7201 aaaaatttgc aggaaaatca tagtaaagac ctcaaaaaat caataaaaaa tttaaaaaat
     7261 tggacaatca catttctgcg taaaatctgc actttttcag taaaaaatac cgattttccg
     7321 tgaaaattct cgatttttaa tcgaaaatct cgaaattttt acaaatttca gcaccaaaat
     7381 cgttgaaaat tctgaaaatt tgaatttttt ttccttgttt tttgagtaaa atttgcactt
     7441 tttcagtaaa gaataccgat tttccgtgaa aattctcggt ttttaatcga aaatctcgaa
     7501 atttttacaa atttcagctc cgaaatagct gaaaattgga caaaaatttc aatttttgcc
     7561 ctgttttttg agtaaaatct gcacttttta gttgaaaaaa aacacggatt ttgtaggaaa
     7621 atttgggaaa aatctccaaa ttttcacaat tttccagtaa aaacagcaat ttttaaaacg
     7681 gtttttaatc aaaaatcttt agaattttcc aaatttcagc accaaaaaag ctaaaaatcg
     7741 ctgaaatttt cgacaaattc caaaaacatt ttcagaagga cgacggaaac tacaagaccc
     7801 gtgccgaggt caagttcgag ggagacaccc tcgtcaaccg tatcgagctc aagggaatcg
     7861 acttcaagga ggacggaaac atcctcggac acaagctcga gtacaactac aactcccaca
     7921 acgtctacat catggccgac aagcaaaaga acggaatcaa ggtcaacttc aaggtaagtt
     7981 cttttttccg agagaaaaac gctgaaaaat gccgaaaatt ttgaatttgc aggcttttta
     8041 ggtgcaaaag tacggaaaaa tcggaaaaaa atcaggaaaa catggggaaa atccaatttt
     8101 ccagaaaaaa tgtttgaaaa aattgaaaac ttttgttttt ttagtaataa agttttaaaa
     8161 aatggcaaaa atagctgaaa acagcgaaaa aaaaaaatgt ttttttcagg aaaaaatttg
     8221 caaaaaaatg aagagcaaca ataattccct tttttttctg tgtttttttc ggaaaaattg
     8281 agatcgaaaa cgaaaaacag cgaaaattcc caattttccc agtttttaaa acattttttt
     8341 gtaaaaattt ggaaaaaacg agttttttca gaaaaaaatg tgaaaaaaaa ggtaaattac
     8401 caatttatgc ttttttttcg atcgagtaaa aaatcgattt ttccaagatt tttcgcgaaa
     8461 aattcaaaaa aaaagaacga aaattctcga ttttctactt tttttcgaaa aaaaaaggaa
     8521 attatagatt tttccaagat ttttcgcgaa aaaattcgaa aaaaatgttt ttttcgaaaa
     8581 tttcagattt tctaattttt tcagaagaat tcgaaaaaaa aaatcaaaaa attcgcaatt
     8641 tttagatttt tttaaaatct caattttcta taattatttt cctaaaaaaa tgtaattaaa
     8701 aaaccgctaa aaacggtaaa ttccaaattt ttgcattttt tcaaaaaaaa tttgaaaaaa
     8761 aaaatcagaa aattctcaaa tctattccct ttttttaaaa tttttcccaa accaaaaaaa
     8821 aaaacggtaa attttcaaat ttagcatttt tccaaaatta aacataaaaa aaaacgcaaa
     8881 tttccaaatt tcgttttttt ttcaaaaaag ttgaaaaatc cagaaaatac cctcttgtcc
     8941 atgaaaaaat acgaaaattt tcaataaaaa atcaatttct tcttaaaaaa ttaattctcg
     9001 atttttccat gaaaaatacg aaaaattcca ataaaaatca actattttaa aaatttgccg
     9061 ttgtaatttt cagatccgtc acaacatcga ggacggatcc gtccaactcg ccgaccacta
     9121 ccaacaaaac accccaatcg gagacggacc agtcctcctc ccagacaacc actacctctc
     9181 cacccaatcc gccctctcca aggtaagttc atacaatttt ctggagaaaa atattttaaa
     9241 aaataacgaa aatggaccga atttttggag aaaattttaa aaaaaacgac atttttccca
     9301 aaaaattgaa aaatttccga aaaaacgttc aaaattttca aaaaaggagc atttttcgaa
     9361 aaaaaaaacg ataatacgga aattttttga ttttccagtt tttttctcaa agaaaatcgg
     9421 aaaaaggttt tttttcttta aaaacgtcca aaatttttcg aaaaaagaga aaattttcga
     9481 ttttccgatt tttttcagaa aaaaattgaa aaaaaaagaa attttccgat tttctcagag
     9541 aaagacgaaa agacgatatt tttcggaaaa aagttgaaaa atcaaaaaaa aaaccgaaaa
     9601 acggccgaaa ttttctaaaa attggaaata ttgtcgattt ttcgaaaaaa aagagtcaaa
     9661 agttttcaaa aaaatatatt ttttcgattt tccgtttttt tttttcagta aacattgaaa
     9721 caaacgaaat ttttccaaaa aaatccaatt attttcccaa atatcgaaat atttttgatt
     9781 ttccgttttt ttcagaaaaa agttaaaaaa aaaagggaaa attaagattt tcctgatttt
     9841 ttttgagaaa aaatcgataa aaattgaata ttctggaaaa aacctttaaa aaattcgaat
     9901 taaatcgaaa aacgtccaaa attttctaaa aaattgaaat tttttttgaa aaaaaacgga
     9961 aaaatttaga tttcccgact tttttcaatg aaaaatcgaa ttttcgttga cttttcatta
    10021 aaaaatccaa acgcattttt ttccactaaa atctcgaaaa agtgtcatat tgcaccatat
    10081 tgtttccaca ttcatattat tttgagctaa atcaggtgat tttctgtatt ttgatactaa
    10141 tttctatttt tagaaaaaat gtgggaacaa tatggtgcaa tatgacattt tttcgagatt
    10201 ttagtggaga aaaaatgcgt ttggaaacga attttataat aaaaagtcaa cgaaaattcg
    10261 atttttcatg gaaaaatagg gaaatcgaaa acaaatttgc ttttttttca aaaaaaaaaa
    10321 cggaaattcg aaatttttcc gttctttcaa aaaaaaaatc gatttttttg gaaatttttt
    10381 ttttcaaatt tttaaaaaga atttttcagg acccaaacga gaagcgtgac cacatggtcc
    10441 tcctcgagtt cgtcaccgcc gccggaatca cccacggaat ggacgagctc tacaagtccc
    10501 gtcgtcgtaa ggccaaccca accaagctct ccgagaacgc caagaagctc gccaaggagg
    10561 tcgagaacaa cccagctttc ttgtacaaag tgggataaat gcaaaatcct ttcaagcatt
    10621 cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg
    10681 cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg
    10741 aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt tcacaatctc tcttcaattc
    10801 cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta
    10861 ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag
    10921 tctcacaact ttattataca tagttgataa ttcactggcc gtcgttttac actcgagacg
    10981 tacggtgcgc gcgatgcatt cgaagatctg cccactagtg agtcgtatta taagtgcaag
    11041 taagatcagt gtttgtttcg atatcagtct gtttcgtaac ggtcttctgt ataactacaa
    11101 aaaagaataa aaaaccgtat cacaactgag aaaaaagaca aaacttattg aacagtgttc
    11161 ctttcaaaaa attattttgt aaaaaatcga gtttttctcc aaaataacgc acgcaatcaa
    11221 aaacaagaaa actaacgtgt tctcctgggc gtggaactcc ttatcaagat taccattatt
    11281 gtctgtcaga attgatattt caattatttc atatctagac aaacccgggt gtcttgcatt
    11341 tattagtact gtgagtttac atacaaacga ggcttactca gataacatag agtcacctct
    11401 aattagcaaa aacccatttc taattagcta tttcgaccag ttctaaagtc accgtaatta
    11461 gaaatggttt ttgtagcttt tctaattaga aatgaccccg agataagtga aatatggcat
    11521 tttctgggtt ttctagtaag aaatgatccc gcacgtagtg attccgcgtt acaagccaaa
    11581 ttcaaattaa aaaagtatat tacacaattc aagttacatt attttcagga aacaatggcg
    11641 aagagcaaag cagagattca aaaggaggaa agagacaaca gatatgaacg aagacttctt
    11701 gaaaagagaa agagacatga ggtgagtaac catgataata aataatcaag taagggctat
    11761 agatttataa attctggagt taacagtttc gaaaaaaagt agttttcata tgtgcgaagg
    11821 aatatcaaaa ataggggaat agcctaaacg agtgagaaaa ttaaatgttt gttatttttt
    11881 caaaaatgtc tgcagggccg ttatcctttc aaatgcactc tatcaaacat agatcatatc
    11941 catcatatct tatccagaat ttatatcata tccaagattg agagcgtttc aaggaaaatc
    12001 tcaaacaacc atacaatttt gtagatcgac aacaaggcct ccacttcttc atcgatggcc
    12061 tctgcagcac ctgcaccacc aagtttgtat ttttcatatt aatataaagt gagccacgca
    12121 tagtcgtttt taggattttt gccagatcga tgtcgcgcaa acagactgtt tttttttctt
    12181 tgaaagccgt gcgtgacatg ggccacggat tgtaacgccg cgctggatgg ataaaaattt
    12241 attcagtttt ttagccttta caaaatatgg ttcgactact ttctttattt ttacattttt
    12301 ataggtgctc attgagctga gatattttgg gtataatcgg aaaatcgctg attttggtca
    12361 tttttctgca ctaaagatta cacaaaaagt tcagtattta gattcattgt tgaaaattgg
    12421 ggatgatcta cagatacata tcgccgtgtt caaaaacaca cgtgacgtcg tgactgggaa
    12481 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    12541 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    12601 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
    12661 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
    12721 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
    12781 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
    12841 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
    12901 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    12961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    13021 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    13081 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    13141 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    13201 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    13261 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    13321 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    13381 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    13441 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    13501 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    13561 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    13621 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    13681 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    13741 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    13801 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    13861 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    13921 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    13981 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    14041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    14101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    14161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    14221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    14281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    14341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    14401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    14461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    14521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    14581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    14641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggtcttt
    14701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    14761 attaccgcct ttgagtgagc tgataccagc ggataacaat ttcaca
//
